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Multicenter interlaboratory study of routine systems for the susceptibility testing of temocillin using a challenge panel of multidrug-resistant strains.
Deckers, Corentin; Bélik, Florian; Denis, Olivier; Montesinos, Isabel; Bogaerts, Pierre; Boelens, Jerina; Brassinne, Laetitia; Descy, Julie; Desmet, Stefanie; Gils, Sarah; Lissoir, Bénédicte; Magerman, Koen; Matheeussen, Veerle; Meex, Cécile; Rodriguez Villalobos, Hector; Van den Abeele, Anne Marie; Vernelen, Kris; Ceyssens, Pieter-Jan; Huang, Te-Din.
Afiliación
  • Deckers C; Laboratory of Clinical Microbiology, CHU UCL Namur, UCLouvain, Site Godinne, Avenue Gaston Therasse, 1, 5530, Yvoir, Belgium. corentin.deckers@uclouvain.be.
  • Bélik F; Laboratory of Clinical Microbiology, CHU UCL Namur, UCLouvain, Site Godinne, Avenue Gaston Therasse, 1, 5530, Yvoir, Belgium.
  • Denis O; Laboratory of Clinical Microbiology, CHU UCL Namur, UCLouvain, Site Godinne, Avenue Gaston Therasse, 1, 5530, Yvoir, Belgium.
  • Montesinos I; Laboratory of Clinical Microbiology, CHU UCL Namur, UCLouvain, Site Godinne, Avenue Gaston Therasse, 1, 5530, Yvoir, Belgium.
  • Bogaerts P; Laboratory of Clinical Microbiology, CHU UCL Namur, UCLouvain, Site Godinne, Avenue Gaston Therasse, 1, 5530, Yvoir, Belgium.
  • Boelens J; Laboratory of Clinical Microbiology, UZ Gent, Ghent, Belgium.
  • Brassinne L; Laboratory of Clinical Microbiology, Cliniques de L'Europe, Brussels, Belgium.
  • Descy J; Laboratory of Clinical Microbiology, Clinique André Renard, Herstal, Belgium.
  • Desmet S; Laboratory of Clinical Microbiology, UZ Leuven, Louvain, Belgium.
  • Gils S; Laboratory of Clinical Microbiology, Medisch Centrum Huisartsen, Louvain, Belgium.
  • Lissoir B; Laboratory of Clinical Microbiology, Grand Hôpital de Charleroi, Charleroi, Belgium.
  • Magerman K; Laboratory of Clinical Microbiology, Jessa Ziekenhuis, Hasselt, Belgium.
  • Matheeussen V; Laboratory of Clinical Microbiology, UZ Antwerp, Antwerp, Belgium.
  • Meex C; Laboratory of Clinical Microbiology, CHU de Liège, Liège, Belgium.
  • Rodriguez Villalobos H; Laboratory of Clinical Microbiology, Cliniques Universitaires Saint-Luc, UCLouvain, Brussels, Belgium.
  • Van den Abeele AM; Laboratory of Clinical Microbiology, AZ Sint-Lucas, Ghent, Belgium.
  • Vernelen K; Quality of Laboratories, Sciensano, Brussels, Belgium.
  • Ceyssens PJ; Unit of Human Bacterial Diseases, Sciensano, Brussels, Belgium.
  • Huang TD; Laboratory of Clinical Microbiology, CHU UCL Namur, UCLouvain, Site Godinne, Avenue Gaston Therasse, 1, 5530, Yvoir, Belgium.
Eur J Clin Microbiol Infect Dis ; 42(12): 1477-1483, 2023 Dec.
Article en En | MEDLINE | ID: mdl-37870713
Accurate susceptibility result of temocillin (TMO) is important for treating infections caused by multidrug-resistant Enterobacterales. This multicenter study aimed to investigate the performance of routine temocillin testing assays against Enterobacterales challenging strains. Forty-seven selected clinical isolates were blindly analyzed by 12 Belgian laboratories using VITEK® 2 (n = 5) and BD Phoenix™ (n = 3) automated systems, ETEST® gradient strip (n = 3), and disk (3 brands) diffusion method (DD; n = 6) for temocillin susceptibility using standardized methodology. Results were interpreted using EUCAST 2023 criteria and compared to the broth microdilution (BMD; Sensititre™ panel) method used as gold standard. Methods' reproducibility was assessed by testing 3 reference strains in triplicate. A total of 702 organism-drug results were obtained against 33 TMO-susceptible and 14 TMO-resistant isolates. Excluding Proteae species (P. mirabilis and M. morganii), the essential agreement rates were excellent (91.5-100%) for all MIC-based methods. The highest category agreement was achieved by ETEST® (97.5%) followed by VITEK® 2 (93.2%), disk diffusion (91.6%), and BD Phoenix™ (88.5%). BD Phoenix™ and paper disk diffusion overcalled resistance (11.5% and 6.8% of major discrepancies, respectively), while ROSCO tablets diffusion and VITEK® 2 generated higher very major discrepancies (7.1% and 4.2% respectively). Inter-assay reproducibility was unsatisfactory using recommended E. coli ATCC 25922 strain but was excellent with E. coli ATCC 35218 and K. pneumoniae ATCC 700603 strains. This interlaboratory study suggests that routine testing methods provide accurate and reproducible TMO categorization results except for Proteae species.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Penicilinas / Escherichia coli / Antibacterianos Límite: Humans Idioma: En Revista: Eur J Clin Microbiol Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Penicilinas / Escherichia coli / Antibacterianos Límite: Humans Idioma: En Revista: Eur J Clin Microbiol Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Alemania