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Photobiological modulation of hepatoma cell lines and hepatitis B subviral particles secretion in response to 650 nm low level laser treatment.
Al-Toukhy, Ghada M; Suef, Reda A; Hassan, Sarah; Farag, Mohamed M S; El-Tayeb, Tarek A; Mansour, Mohamed T M.
Afiliación
  • Al-Toukhy GM; Department of Virology and Immunology, Children's Cancer Hospital, Cairo, 57357, Egypt. gh.mn2013@gmail.com.
  • Suef RA; Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Cairo, 11884, Egypt.
  • Hassan S; Pathology and Electron Microscopy, Theodor Bilharz Research Institute, Giza, Egypt.
  • Farag MMS; Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Cairo, 11884, Egypt.
  • El-Tayeb TA; Biomedical Research Department, Armed Forces College of Medicine, Cairo, Egypt.
  • Mansour MTM; National Institute of Laser Enhanced Science (NILES), Cairo University, Cairo, Egypt.
J Egypt Natl Canc Inst ; 35(1): 33, 2023 Oct 23.
Article en En | MEDLINE | ID: mdl-37870653
BACKGROUND: Chronic hepatitis B virus (HBV) infection is a serious global health concern, with an increased incidence and risk of developing cirrhosis and hepatocellular carcinoma (HCC). Patients chronically infected with HBV are likely to experience chronic oxidative stress, leading to mitochondrial dysfunction. Photobiomodulation is induced by the absorption of low-level laser therapy (LLLT) with a red or infrared laser by cytochrome C oxidase enzyme, resulting in mitochondrial photoactivation. Although it is widely used in clinical practice, the use of LLL as adjuvant therapy for persistent HBV infection is uncommon. This study aimed to investigate the effect of LLLT dosage from 2 J/cm2 to 10 J/cm2 of red diode laser (650 nm) on both hepatoma cell lines (HepG2.2.15 [integrated HBV genome stable cell model] and non-integrated HepG2), with a subsequent impact on HBVsvp production. METHODS: The present study evaluated the effects of different fluences of low-level laser therapy (LLLT) irradiation on various aspects of hepatoma cell behavior, including morphology, viability, ultrastructure, and its impact on HBVsvp synthesis. RESULTS: In response to LLLT irradiation, we observed a considerable reduction in viability, proliferation, and HBVsvp production in both hepatoma cell lines HepG2.2.15 and HepG2. Ultrastructural modification of mitochondria and nuclear membranes: This effect was dose, cell type, and time-dependent. CONCLUSIONS: The use of LLLT may be a promising therapy for HCC and HBV patients by reducing cell proliferation, HBVsvp production, and altering mitochondrial and nuclear structure involved in cellular death inducers. Further research is required to explore its clinical application.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma Hepatocelular / Hepatitis B Crónica / Hepatitis B / Neoplasias Hepáticas Límite: Humans Idioma: En Revista: J Egypt Natl Canc Inst Asunto de la revista: NEOPLASIAS Año: 2023 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma Hepatocelular / Hepatitis B Crónica / Hepatitis B / Neoplasias Hepáticas Límite: Humans Idioma: En Revista: J Egypt Natl Canc Inst Asunto de la revista: NEOPLASIAS Año: 2023 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Reino Unido