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Human umbilical cord mesenchymal stem cells alleviated TNBS-induced colitis in mice by restoring the balance of intestinal microbes and immunoregulation.
Fu, Yanxia; Zhang, Chen; Xie, Hui; Wu, Zisheng; Tao, Yurong; Wang, Ziyu; Gu, Meng; Wei, Panjian; Lin, Shuye; Li, Ruoran; He, Yuqi; Sheng, Jianqiu; Xu, Junfeng; Wang, Jinghui; Pan, Yuanming.
Afiliación
  • Fu Y; Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing 100069, China.
  • Zhang C; Chinese PLA General Hospital and Medical School, Beijing 100853, China; Department of Gastroenterology, The Seventh Medical Center of Chinese PLA General Hospital, Beijing 100700, China.
  • Xie H; Department of Gastroenterology, The Seventh Medical Center of Chinese PLA General Hospital, Beijing 100700, China.
  • Wu Z; Chinese PLA General Hospital and Medical School, Beijing 100853, China.
  • Tao Y; Department of Gastroenterology, The Seventh Medical Center of Chinese PLA General Hospital, Beijing 100700, China.
  • Wang Z; Cancer Research Center, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
  • Gu M; Cancer Research Center, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
  • Wei P; Cancer Research Center, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
  • Lin S; Cancer Research Center, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
  • Li R; Chinese PLA General Hospital and Medical School, Beijing 100853, China.
  • He Y; Department of Gastroenterology, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
  • Sheng J; Department of Gastroenterology, The Seventh Medical Center of Chinese PLA General Hospital, Beijing 100700, China.
  • Xu J; Department of Gastroenterology, The First Medical Center of Chinese PLA General Hospital, Beijing 100853, China. Electronic address: xujunfeng@301hospital.com.cn.
  • Wang J; Cancer Research Center, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China. Electronic address: jinghuiwang2006@163.com.
  • Pan Y; Cancer Research Center, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China. Electronic address: peterfpan2020@mail.ccmu.edu.cn.
Life Sci ; 334: 122189, 2023 Dec 01.
Article en En | MEDLINE | ID: mdl-37865178
AIMS: Human umbilical cord mesenchymal stem cells (HUMSCs) have been documented to be effective for several immune disorders including inflammatory bowel diseases (IBD). However, it remains unclear how HUMSCs function in regulating immune responses and intestinal flora in the trinitrobenzene sulfonic acid (TNBS)-induced IBD model. MATERIALS AND METHODS: We assessed the regulatory effects of HUMSCs on the gut microbiota, T lymphocyte subpopulations and related immune cytokines in the TNBS-induced IBD model. The mice were divided into the normal, TNBS, and HUMSC-treated groups. The effect of HUMSCs was evaluated by Hematoxylin and Eosin (H&E) staining, fluorescence-activated cell sorting (FACS), and enzyme-linked immunosorbent assay (ELISA) analyses. Metagenomics Illumina sequencing was conducted for fecal samples. KEY FINDINGS: We demonstrated that the disease symptoms and pathological changes in the colon tissues of TNBS-induced colitis mice were dramatically ameliorated by HUMSCs, which improved the gut microbiota and rebalanced the immune system, increasing the abundance of healthy bacteria (such as Lactobacillus murinus and Lactobacillus johnsonii), the Firmicutes/Bacteroidetes ratio, and the proportion of Tregs; the Th1/Th17 ratio was decreased. Consistently, the expression levels of IFN-γ and IL-17 were significantly decreased, and transforming growth factor-ß1 (TGF-ß1) levels were significantly increased in the plasma of colitis mice HUMSC injection. SIGNIFICANCE: Our experiment revealed that HUMSCs mitigate acute colitis by regulating the rebalance of Th1/Th17/Treg cells and related cytokines and remodeling the gut microbiota, providing potential future therapeutic targets in IBD.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades Inflamatorias del Intestino / Colitis / Células Madre Mesenquimatosas Límite: Animals / Humans Idioma: En Revista: Life Sci Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades Inflamatorias del Intestino / Colitis / Células Madre Mesenquimatosas Límite: Animals / Humans Idioma: En Revista: Life Sci Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos