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Flow injection analysis coupled with photoelectrochemical immunoassay for simultaneous detection of anti-SARS-CoV-2-spike and anti-SARS-CoV-2-nucleocapsid antibodies in serum samples.
Wang, Ruimin; Wang, Shuai; Guo, Wanze; Zhang, Tiantian; Kang, Qing; Wang, Pengcheng; Zhou, Feimeng; Yang, Lixia.
Afiliación
  • Wang R; Institute of Surface Analysis and Chemical Biology, University of Jinan, Jinan, Shandong, 250022, PR China.
  • Wang S; Institute of Surface Analysis and Chemical Biology, University of Jinan, Jinan, Shandong, 250022, PR China.
  • Guo W; Institute of Surface Analysis and Chemical Biology, University of Jinan, Jinan, Shandong, 250022, PR China.
  • Zhang T; University Hospital, University of Jinan, Jinan, Shandong, 250022, PR China.
  • Kang Q; Institute of Surface Analysis and Chemical Biology, University of Jinan, Jinan, Shandong, 250022, PR China. Electronic address: ila_kangq@ujn.edu.cn.
  • Wang P; Institute of Surface Analysis and Chemical Biology, University of Jinan, Jinan, Shandong, 250022, PR China. Electronic address: ila_wangpc@ujn.edu.cn.
  • Zhou F; School of Life Sciences, Tiangong University, Tianjin, 300387, PR China.
  • Yang L; Key Laboratory of Jiangxi Province for Persistent Pollutants Control and Resources Recycle, Nanchang Hangkong University, Nanchang, 330063, PR China.
Anal Chim Acta ; 1280: 341857, 2023 Nov 01.
Article en En | MEDLINE | ID: mdl-37858551
A thin-layer flow cell of low internal volume (12 µL) is incorporated in a flow injection analysis (FIA) system for simultaneous and real-time photoelectrochemical (PEC) immunoassay of anti-SARS-CoV-2 spike 1 (S1) and anti-SARS-CoV-2 nucleocapsid (N) antibodies. Covalent linkage of S1 and N proteins to two separate polyethylene glycol (PEG)-covered gold nanoparticles (AuNPs)/TiO2 nanotube array (NTA) electrodes affords 10 consecutive analyses with surface regenerations in between. An indium tin oxide (ITO) allows visible light to impinge onto the two electrodes. The detection limits for anti-S1 and anti-N antibodies were estimated to be 177 and 97 ng mL-1, respectively. Such values compare well with those achieved with other reported methods and satisfy the requirement for screening convalescent patients with low antibody levels. Additionally, our method exhibits excellent intra-batch (RSD = 1.3%), inter-batch (RSD = 3.4%), intra-day (RSD = 1.0%), and inter-day (RSD = 1.6%) reproducibility. The obviation of an enzyme label and continuous analysis markedly decreased the assay cost and duration, rendering this method cost-effective. The excellent anti-fouling property of PEG enables accuracy validation by comparing our PEC immunoassays of patient sera to those of ELISA. In addition, the simultaneous detection of two antibodies holds great potential in disease diagnosis and immunity studies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas del Metal / COVID-19 Límite: Humans Idioma: En Revista: Anal Chim Acta Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas del Metal / COVID-19 Límite: Humans Idioma: En Revista: Anal Chim Acta Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos