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Identification of OLA1 as a Novel Protein Target of Vitexin to Ameliorate Dextran Sulfate Sodium-Induced Colitis with Tissue Thermal Proteome Profiling.
Li, Nan; Wang, Ruiyan; Li, Wenwen; Du, Qian; Deng, Zeyuan; Fan, Yawei; Zheng, Liufeng.
Afiliación
  • Li N; State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, Jiangxi, China.
  • Wang R; State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, Jiangxi, China.
  • Li W; State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, Jiangxi, China.
  • Du Q; State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, Jiangxi, China.
  • Deng Z; State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, Jiangxi, China.
  • Fan Y; Institute for Advanced Study, University of Nanchang, Nanchang 330031, Jiangxi, China.
  • Zheng L; State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, Jiangxi, China.
J Agric Food Chem ; 71(43): 16057-16066, 2023 Nov 01.
Article en En | MEDLINE | ID: mdl-37856434
Vitexin, which exists in various medicinal plants and food sources, has recently received increasing attention because of its anti-inflammatory properties. This study aims to identify the protein target of vitexin that ameliorates dextran sulfate sodium (DSS)-induced colitis. The results showed that vitexin not only alleviated the clinical symptoms and colonic damage in mice with DSS-induced colitis but also suppressed the colonic production of inflammatory cytokines (IL-1ß, IL-6, ICAM, and VCAM) and enhanced the expression of barrier-associated proteins (ZO-1, Occludin, and E-cadherin). Based on tissue thermal proteome profiling (Tissue-TPP) and molecular docking, OLA1 was creatively identified as a potential protein target for vitexin. Further siRNA-mediated knockdown of the OLA1 gene in Caco-2 cells demonstrated the ability of OLA1 to increase Nrf2 protein expression and, thus, mediated the anti-inflammatory effects of vitexin. Interaction of the OLA1-vitexin complex with Keap1 protein to disrupt the Keap1-Nrf2 interaction may be required for activating Nrf2. Our findings revealed a novel role for OLA1 as a protein target of vitexin that contributes to its anti-inflammatory action by activating Nrf2, which may provide a promising molecular mechanism for novel therapeutic strategies to treat colitis and the associated systemic inflammation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colitis Ulcerosa / Colitis Límite: Animals / Humans Idioma: En Revista: J Agric Food Chem Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colitis Ulcerosa / Colitis Límite: Animals / Humans Idioma: En Revista: J Agric Food Chem Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos