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miR-28-based combination therapy impairs aggressive B cell lymphoma growth by rewiring DNA replication.
Fuertes, Teresa; Álvarez-Corrales, Emigdio; Gómez-Escolar, Carmen; Ubieto-Capella, Patricia; Serrano-Navarro, Álvaro; de Molina, Antonio; Méndez, Juan; Ramiro, Almudena R; de Yébenes, Virginia G.
Afiliación
  • Fuertes T; B Cell Biology Laboratory Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain.
  • Álvarez-Corrales E; Department of Immunology, Ophthalmology and ENT, Universidad Complutense de Madrid; Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), Madrid, Spain.
  • Gómez-Escolar C; B Cell Biology Laboratory Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain.
  • Ubieto-Capella P; DNA replication Group. Centro Nacional de Investigaciones Oncológicas (CNIO), Madrid, Spain.
  • Serrano-Navarro Á; B Cell Biology Laboratory Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain.
  • de Molina A; Comparative Medicine Unit. Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain.
  • Méndez J; DNA replication Group. Centro Nacional de Investigaciones Oncológicas (CNIO), Madrid, Spain.
  • Ramiro AR; B Cell Biology Laboratory Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain. aramiro@cnic.es.
  • de Yébenes VG; Department of Immunology, Ophthalmology and ENT, Universidad Complutense de Madrid; Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), Madrid, Spain. vgarciay@ucm.es.
Cell Death Dis ; 14(10): 687, 2023 10 18.
Article en En | MEDLINE | ID: mdl-37852959
Diffuse large B cell lymphoma (DLBCL) is the most common aggressive B cell lymphoma and accounts for nearly 40% of cases of B cell non-Hodgkin lymphoma. DLBCL is generally treated with R-CHOP chemotherapy, but many patients do not respond or relapse after treatment. Here, we analyzed the therapeutic potential of the tumor suppressor microRNA-28 (miR-28) for DLBCL, alone and in combination with the Bruton's tyrosine kinase inhibitor ibrutinib. Combination therapy with miR-28 plus ibrutinib potentiated the anti-tumor effects of monotherapy with either agent by inducing a specific transcriptional cell-cycle arrest program that impairs DNA replication. The molecular actions of miR-28 and ibrutinib synergistically impair DNA replication by simultaneous inhibition of origin activation and fork progression. Moreover, we found that downregulation of the miR-28-plus-ibrutinib gene signature correlates with better survival of ABC-DLBCL patients. These results provide evidence for the effectiveness of a new miRNA-based ibrutinib combination therapy for DLBCL and unveil the miR-28-plus-ibrutinib gene signature as a new predictor of outcome in ABC-DLBCL patients.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfoma de Células B Grandes Difuso / MicroARNs Límite: Humans Idioma: En Revista: Cell Death Dis Año: 2023 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfoma de Células B Grandes Difuso / MicroARNs Límite: Humans Idioma: En Revista: Cell Death Dis Año: 2023 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido