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BBX21 integrates brassinosteroid biosynthesis and signalling in the inhibition of hypocotyl growth under shade.
Gómez-Ocampo, Gabriel; Crocco, Carlos D; Cascales, Jimena; Oklestkova, Jana; Tarkowská, Danuse; Strnad, Miroslav; Mora-Garcia, Santiago; Pruneda-Paz, José L; Blazquez, Miguel A; Botto, Javier F.
Afiliación
  • Gómez-Ocampo G; IFEVA (CONICET-UBA), Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, Ciudad Autónoma de Buenos Aires, C1417DSE, Argentina.
  • Crocco CD; IFEVA (CONICET-UBA), Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, Ciudad Autónoma de Buenos Aires, C1417DSE, Argentina.
  • Cascales J; IFEVA (CONICET-UBA), Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, Ciudad Autónoma de Buenos Aires, C1417DSE, Argentina.
  • Oklestkova J; Laboratory of Growth Regulators, Palacký University & Institute of Experimental Botany, The Czech Academy of Sciences, Slechtitelu 27, CZ-78371 Olomouc, Czech Republic.
  • Tarkowská D; Laboratory of Growth Regulators, Palacký University & Institute of Experimental Botany, The Czech Academy of Sciences, Slechtitelu 27, CZ-78371 Olomouc, Czech Republic.
  • Strnad M; Laboratory of Growth Regulators, Palacký University & Institute of Experimental Botany, The Czech Academy of Sciences, Slechtitelu 27, CZ-78371 Olomouc, Czech Republic.
  • Mora-Garcia S; Fundación Instituto Leloir, IIBBA-CONICET, Avenida Patricias Argentinas 435, Ciudad Autónoma de Buenos Aires, C1405BWE, Argentina.
  • Pruneda-Paz JL; Section of Cell and Developmental Biology, Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA.
  • Blazquez MA; Instituto de Biología Molecular y Celular de Plantas, CSIC-Universitat Politècnica de València, C/Ingeniero Fausto Elio s/n, Valencia, 46022, Spain.
  • Botto JF; IFEVA (CONICET-UBA), Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, Ciudad Autónoma de Buenos Aires, C1417DSE, Argentina.
Plant Cell Physiol ; 2023 Oct 16.
Article en En | MEDLINE | ID: mdl-37847120
B-Box-containing zinc finger transcription factors (BBX) are involved in light-mediated growth, affecting processes such as hypocotyl elongation in Arabidopsis thaliana. However, the molecular and hormonal framework that regulates plant growth through BBX proteins is incomplete. Here, we demonstrate that BBX21 inhibits the hypocotyl elongation through the brassinosteroid (BR) pathway. BBX21 reduces the sensitivity to 24-epiBL, a synthetic active BR, principally at very-low concentrations in simulated shade. The biosynthesis profile of BRs showed that two active BR -brassinolide (BL) and 28-homobrassinolide (28-homoBL)- and 8 of 11 intermediates can be repressed by BBX21 under white light (WL) or simulated shade. Furthermore, BBX21 represses the expression of CYTOCHROME P450 90B1 (DWF4/CYP90B1), BRASSINOSTEROID-6-OXIDASE 1 (BR6OX1, CYP85A1) and BR6OX2 (CYP85A2) genes involved in the BR biosynthesis in WL while specifically promoting DWF4 and PHYB ACTIVATION TAGGED SUPPRESSOR 1 (CYP2B1/BAS1) expression in WL supplemented with far-red (WL+FR), a treatment that simulates shade. In addition, BBX21 represses BR signalling genes such as PACLOBUTRAZOL RESISTANCE1 (PRE1), PRE3 and ARABIDOPSIS MYB-LIKE 2 (MYBL2), and auxin-related and expansin genes, such as INDOLE-3-ACETIC ACID INDUCIBLE 1 (IAA1), IAA4 and EXPANSIN 11 (EXP11) in short-term shade. By a genetic approach we found that BBX21 acts genetically upstream of BRASSINAZOLE-RESISTANT 1 (BZR1) for the promotion of DWF4 and BAS1 gene expression in shade. We propose that BBX21 integrates the BR homeostasis and shade-light signalling allowing the fine-tuning of hypocotyl elongation in Arabidopsis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Plant Cell Physiol Asunto de la revista: BOTANICA Año: 2023 Tipo del documento: Article País de afiliación: Argentina Pais de publicación: Japón
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Plant Cell Physiol Asunto de la revista: BOTANICA Año: 2023 Tipo del documento: Article País de afiliación: Argentina Pais de publicación: Japón