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Measurement of Urinary Gc-Globulin by a Fluorescence ELISA Technique: Method Validation and Clinical Evaluation in Septic Patients-A Pilot Study.
Koszegi, Tamás; Horváth-Szalai, Zoltán; Ragán, Dániel; Kósa, Brigitta; Szirmay, Balázs; Kurdi, Csilla; Kovács, Gábor L; Mühl, Diána.
Afiliación
  • Koszegi T; Department of Laboratory Medicine, Medical School, University of Pécs, 7624 Pécs, Hungary.
  • Horváth-Szalai Z; János Szentágothai Research Center, University of Pécs, 7624 Pécs, Hungary.
  • Ragán D; Hungarian National Laboratory on Reproduction, University of Pécs, 7624 Pécs, Hungary.
  • Kósa B; Department of Laboratory Medicine, Medical School, University of Pécs, 7624 Pécs, Hungary.
  • Szirmay B; János Szentágothai Research Center, University of Pécs, 7624 Pécs, Hungary.
  • Kurdi C; Department of Laboratory Medicine, Medical School, University of Pécs, 7624 Pécs, Hungary.
  • Kovács GL; Department of Laboratory Medicine, Medical School, University of Pécs, 7624 Pécs, Hungary.
  • Mühl D; János Szentágothai Research Center, University of Pécs, 7624 Pécs, Hungary.
Molecules ; 28(19)2023 Sep 29.
Article en En | MEDLINE | ID: mdl-37836706
A major complication of sepsis is the development of acute kidney injury (AKI). In case of acute tubular damage, Gc-globulin, a known serum sepsis marker is increasingly filtrated into the urine therefore, urinary Gc-globulin (u-Gc) levels may predict septic AKI. We developed and validated a competitive fluorescence ELISA method for u-Gc measurement. Serum and urine samples from septic patients were collected in three consecutive days (T1, T2, T3) and data were compared to controls. Intra- and interassay imprecisions were CV < 14% and CV < 20%, respectively, with a recovery close to 100%. Controls and septic patients differed (p < 0.001) in their u-Gc/u-creatinine levels at admission (T1, median: 0.51 vs. 79.1 µg/mmol), T2 (median: 0.51 vs. 57.8 µg/mmol) and T3 (median: 0.51 vs. 55.6 µg/mmol). Septic patients with AKI expressed higher u-Gc/u-creatinine values than those without AKI at T1 (median: 23.6 vs. 136.5 µg/mmol, p < 0.01) and T3 (median: 34.4 vs. 75.8 µg/mmol, p < 0.05). AKI-2 stage patients exhibited more increased u-Gc/u-creatinine levels at T1 (median: 207.1 vs. 53.3 µg/mmol, p < 0.05) than AKI-1 stage individuals. Moderate correlations (p < 0.001) were observed between u-Gc/u-creatinine and se-urea, se-creatinine, se-hsCRP, WBC, u-total protein, u-albumin, u-orosomucoid/u-creatinine, and u-Cystatin C/u-creatinine levels. U-Gc testing may have a predictive value for AKI in septic patients.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sepsis / Lesión Renal Aguda / Globulinas Límite: Humans Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sepsis / Lesión Renal Aguda / Globulinas Límite: Humans Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: Suiza