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GYRB - POLYMERASE CHAIN REACTION AND HISTOPATHOLOGIC CHARACTERISTIC FIGURE POTENTIAL FOR DETERMINING DIAGNOSIS OF TUBERCULOUS LYMPHADENITIS.
Wahyu, Herlambang; Made, Mertaniasih Ni; Soedarsono, Soedarsono; Willy, Sandhika.
Afiliación
  • Wahyu H; Master Program of Tropical Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
  • Made MN; Department of Medical Microbiology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
  • Soedarsono S; Department of Clinical Microbiology, Dr. Soetomo Academic Hospital, Surabaya, Indonesia.
  • Willy S; Tuberculosis Laboratory, Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
Afr J Infect Dis ; 17(2 Suppl): 13-18, 2023.
Article en En | MEDLINE | ID: mdl-37822554
Background: TB lymphadenitis is still a problem that needs serious treatment. In Indonesia, it was reported that 53% of TB cases were extrapulmonary tuberculosis, with the most cases being Lymphadenitis TB, 11.6%. In children, 43% of extrapulmonary tuberculosis cases are TB lymphadenitis. Diagnosis is quite difficult; a method of determining the diagnosis and appropriate comprehensive treatment is required in managing TB Lymphadenitis. Materials and Methods: In this study, 15 fine needle aspiration biopsy aspirate samples were subjected to molecular examination using the gyrB-polymerase chain reaction method and histopathological observations using the smear method with hematoxylin-eosin staining. Observation of preparations using a microscope with a magnification of 200x. Results: The histopathological characteristics of the fine needle aspiration biopsy aspirate showed positive results in 4 out of 15 samples, with epithelioid cells arranged in a characteristic granuloma structure, necrotic debris was visible, and cells joined together to form multinucleated giant cells as an inflammatory response to Mycobacterium tuberculosis complex infection. In this study, 6 out of 15 (40%) were detected to be positive in the diagnosis based on molecular detection using a specific target gene gyrB - polymerase chain reaction . Conclusion: Characteristic features on histopathological examination associated with gyrB - positive polymerase chain reaction on lymphadenitis fine needle aspiration biopsy aspirate samples can be used as a determinant diagnosis of tuberculous lymphadenitis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Afr J Infect Dis Año: 2023 Tipo del documento: Article País de afiliación: Indonesia Pais de publicación: Nigeria
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Afr J Infect Dis Año: 2023 Tipo del documento: Article País de afiliación: Indonesia Pais de publicación: Nigeria