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Enzyme-functionalized alginate microparticles enable anaerobic culture under ambient oxygen.
Williams, Tyrell J; Daboin, Claudia; Kim, Paul; Guo, Fengguang; de Figueiredo, Paul; Alge, Daniel L.
Afiliación
  • Williams TJ; Department of Biomedical Engineering, Texas A&M University, College Station, Texas, USA.
  • Daboin C; Department of Biomedical Engineering, Texas A&M University, College Station, Texas, USA.
  • Kim P; Department of Biomedical Engineering, Texas A&M University, College Station, Texas, USA.
  • Guo F; Department of Microbial Pathogenesis and Immunology, School of Medicine, Texas A&M Health Science Center, Bryan, Texas, USA.
  • de Figueiredo P; Department of Microbial Pathogenesis and Immunology, School of Medicine, Texas A&M Health Science Center, Bryan, Texas, USA.
  • Alge DL; Department of Veterinary Pathobiology, School of Veterinary Medicine, Texas A&M University, College Station, Texas, USA.
Biotechnol Bioeng ; 121(1): 219-227, 2024 01.
Article en En | MEDLINE | ID: mdl-37807712
Methods for culturing oxygen-sensitive cells and organisms under anaerobic conditions are vital to biotechnology research. Here, we report a biomaterial-based platform for anaerobic culture that consists of glucose oxidase (GOX) functionalized alginate microparticles (ALG-GOX), which are designed to deplete dissolved [O2 ] through enzymatic activity. ALG-GOX microparticles were synthesized via a water-in-oil emulsion and had a size of 132.0 ± 51.4 µm. Despite having a low storage modulus, the microparticles remained stable under aqueous conditions due to covalent crosslinking through amide bonds. Enzyme activity was tunable based on the loaded GOX concentration, with a maximum activity of 3.6 ± 0.3 units/mg of microparticles being achieved at an initial loading concentration of 5 mg/mL of GOX in alginate precursor solution. High enzyme activity in ALG-GOX microparticles resulted in rapid oxygen depletion, producing a suitable environment for anaerobic culture. Microparticles loaded with both GOX and catalase (ALG-GOX-CAT) to reduce H2 O2 buildup exhibited sustained activity for potential long-term anaerobic culture. ALG-GOX-CAT microparticles were highly effective for the anaerobic culture of Bacteroides thetaiotaomicron, with 10 mg/mL of ALG-GOX-CAT microparticles supporting the same level of growth in an aerobic environment compared to an anaerobic chamber after 16 h (8.70 ± 0.96 and 10.03 ± 1.03 million CFU, respectively; N.S. p = 0.07). These microparticles could be a valuable tool for research and development in biotechnology.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Cultivo de Célula / Alginatos Idioma: En Revista: Biotechnol Bioeng Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Cultivo de Célula / Alginatos Idioma: En Revista: Biotechnol Bioeng Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos