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Enhancing bovine tuberculosis screening at Dhaka city in Bangladesh: Integrating gamma interferon blood test as ancillary testing with tuberculin skin test.
Islam, Robiul; Islam, Sk Shaheenur; Rumi, Tanzida Begum; Mia, Zakaria; Rahim, Zeaur.
Afiliación
  • Islam R; Department of Microbiology, Jagannath University, Dhaka 1100, Bangladesh. Electronic address: shantorobiul@gmail.com.
  • Islam SS; Department of Livestock Services, Krishi Khamar Sarak, Farmgate, Dhaka 1215, Bangladesh; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
  • Rumi TB; International Centre for Diarrhoeal Disease and Research, Bangladesh (icddr,b), Mohakhali, Dhaka 1215, Bangladesh.
  • Mia Z; Department of Microbiology, Jagannath University, Dhaka 1100, Bangladesh.
  • Rahim Z; International Centre for Diarrhoeal Disease and Research, Bangladesh (icddr,b), Mohakhali, Dhaka 1215, Bangladesh.
Vet Immunol Immunopathol ; 264: 110659, 2023 Oct.
Article en En | MEDLINE | ID: mdl-37801841
Tuberculin skin test (TST) is the standard method for screening of bovine tuberculosis (bTB). However, gamma interferon blood test has been introduced in the bTB control program as an ancillary testing with TST in many countries of the world. The objective of this study was to recommend this screening test as an ancillary testing with TST for field application in Bangladesh. In this study 577 cattle of different age, sex and breeds from twenty nine (29) cattle herds were examined to determine skin response against bTB through single intradermal comparative tuberculin test (SICTT) that comprised of positive (n = 81), inconclusive (n = 44) and negative (n = 452) animals. Of which 74 animals that included positive (n = 63), inconclusive (n = 8) and negative (n = 3) animals were taken under this study. Blood samples were collected in heparinized tube and stimulated overnight with bovine and avian purified protein derivatives (PPDs) for the secretion of gamma interferon, and measured via sandwich ELISA. Cohen's kappa statistics was performed for the evaluation of agreement between the two tests. The agreement obtained between two tests was fair (Kappa agreement, K = 24.0%, 95% CI = 16.9-30.5%, P = 0.037). Of positive (n = 63), inconclusive (n = 8) and negative (n = 3) status of animals at SICTT, 82.54% (n = 52), 62.50% (n = 5), and 33.33% (n = 1) were found to be bTB positive respectively through this ancillary test. This test notably corroborates to TST result. A considerable number of inconclusive TB status animals were found to be positive through this gamma interferon assay. Therefore, this test could be used as an ancillary test with TST to maximize the proportion of bTB estimation in the infected cattle herd for early detection of zoonotic tuberculosis in Bangladesh before transmission at the animal-human interface.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tuberculosis Bovina / Enfermedades de los Bovinos / Mycobacterium bovis Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Animals / Humans País/Región como asunto: Asia Idioma: En Revista: Vet Immunol Immunopathol Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tuberculosis Bovina / Enfermedades de los Bovinos / Mycobacterium bovis Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Animals / Humans País/Región como asunto: Asia Idioma: En Revista: Vet Immunol Immunopathol Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos