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DeoR regulates lincomycin production in Streptomyces lincolnensis.
Zou, Jingyun; Mao, Yue; Hou, Bingbing; Kang, Yajing; Wang, Ruida; Wu, Haizhen; Ye, Jiang; Zhang, Huizhan.
Afiliación
  • Zou J; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
  • Mao Y; Department of Applied Biology, East China University of Science and Technology, Shanghai, 200237, China.
  • Hou B; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
  • Kang Y; Department of Applied Biology, East China University of Science and Technology, Shanghai, 200237, China.
  • Wang R; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
  • Wu H; Department of Applied Biology, East China University of Science and Technology, Shanghai, 200237, China.
  • Ye J; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
  • Zhang H; Department of Applied Biology, East China University of Science and Technology, Shanghai, 200237, China.
World J Microbiol Biotechnol ; 39(12): 332, 2023 Oct 06.
Article en En | MEDLINE | ID: mdl-37801155
Regulators belonging to the DeoR family are widely distributed among the bacteria. Few studies have reported that DeoR family proteins regulate secondary metabolism of Streptomyces. This study explored the function of DeoR (SLINC_8027) in Streptomyces lincolnensis. Deletion of deoR in NRRL 2936 led to an increase in cell growth. The lincomycin production of the deoR deleted strain ΔdeoR was 3.4-fold higher than that of the wild strain. This trait can be recovered to a certain extent in the deoR complemented strain ΔdeoR::pdeoR. According to qRT-PCR analysis, DeoR inhibited the transcription of all detectable genes in the lincomycin biosynthesis cluster and repressed the expression of glnR, bldD, and SLCG_Lrp, which encode regulators outside the cluster. DeoR also inhibited the transcription of itself, as revealed by the XylE reporter. Furthermore, we demonstrated that DeoR bound directly to the promoter region of deoR, lmbA, lmbC-D, lmbJ-K, lmrA, lmrC, glnR, and SLCG_Lrp, by recognizing the 5'-CGATCR-3' motif. This study found that versatile regulatory factor DeoR negatively regulates lincomycin biosynthesis and cellular growth in S. lincolnensis, which expanded the regulatory network of lincomycin biosynthesis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptomyces / Lincomicina Idioma: En Revista: World J Microbiol Biotechnol Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptomyces / Lincomicina Idioma: En Revista: World J Microbiol Biotechnol Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania