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Macrophage depletion attenuates degeneration of spiral ganglion neurons in kanamycin-induced unilateral hearing loss model.
Shimada, Mari Dias; Noda, Masao; Koshu, Ryota; Takaso, Yuji; Sugimoto, Hisashi; Ito, Makoto; Yoshizaki, Tomokazu; Hori, Osamu.
Afiliación
  • Shimada MD; Department of Otolaryngology, Jichi Medical University, Shimotsuke, Tochigi, Japan.
  • Noda M; Department of Pediatric Otolaryngology, Jichi Children's Medical Center Tochigi, Jichi Medical University, Shimotsuke, Tochigi, Japan.
  • Koshu R; Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Ishikawa, Japan.
  • Takaso Y; Department of Neuroanatomy, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Ishikawa, Japan.
  • Sugimoto H; Department of Otolaryngology, Jichi Medical University, Shimotsuke, Tochigi, Japan.
  • Ito M; Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Ishikawa, Japan.
  • Yoshizaki T; Department of Neuroanatomy, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Ishikawa, Japan.
  • Hori O; Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Ishikawa, Japan.
Sci Rep ; 13(1): 16741, 2023 10 05.
Article en En | MEDLINE | ID: mdl-37798459
Pathological conditions in cochlea, such as ototoxicity, acoustic trauma, and age-related cochlear degeneration, induce cell death in the organ of Corti and degeneration of the spiral ganglion neurons (SGNs). Although macrophages play an essential role after cochlear injury, its role in the SGNs is limitedly understood. We analyzed the status of macrophage activation and neuronal damage in the spiral ganglion after kanamycin-induced unilateral hearing loss in mice. The number of ionized calcium-binding adapter molecule 1 (Iba1)-positive macrophages increased 3 days after unilateral kanamycin injection. Macrophages showed larger cell bodies, suggesting activation status. Interestingly, the number of activating transcription factor 3 (ATF3)-positive-neurons, an indicator of early neuronal damage, also increased at the same timing. In the later stages, the number of macrophages decreased, and the cell bodies became smaller, although the number of neuronal deaths increased. To understand their role in neuronal damage, macrophages were depleted via intraperitoneal injection of clodronate liposome 24 h after kanamycin injection. Macrophage depletion decreased the number of ATF3-positive neurons at day 3 and neuronal death at day 28 in the spiral ganglion following kanamycin injection. Our results suggest that suppression of inflammation by clodronate at early timing can protect spiral ganglion damage following cochlear insult.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ganglio Espiral de la Cóclea / Pérdida Auditiva Unilateral Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Sci Rep Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ganglio Espiral de la Cóclea / Pérdida Auditiva Unilateral Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Sci Rep Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido