The miR156/SPL12 module orchestrates fruit colour change through directly regulating ethylene production pathway in blueberry.

Li, Hongxue; Wang, Shouwen; Zhai, Lulu; Cui, Yuhai; Tang, Guiliang; Huo, Junwei; Li, Xuyan; Bian, Shaomin

Li, Hongxue; Wang, Shouwen; Zhai, Lulu; Cui, Yuhai; Tang, Guiliang; Huo, Junwei; Li, Xuyan; Bian, Shaomin.

Afiliación

Li H; College of Plant Science, Jilin University, Changchun, China.
Wang S; College of Plant Science, Jilin University, Changchun, China.
Zhai L; College of Plant Science, Jilin University, Changchun, China.
Cui Y; Agriculture and Agri-Food Canada, London Research and Development Centre, London, ON, Canada.
Tang G; Department of Biology, Western University, London, ON, Canada.
Huo J; Department of Biological Sciences, Life Science and Technology Institute, Michigan Technological University, Houghton, MI, USA.
Li X; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin, China.
Bian S; College of Plant Science, Jilin University, Changchun, China.

Plant Biotechnol J ; 22(2): 386-400, 2024 Feb.

Article en En | MEDLINE | ID: mdl-37797061

RESUMEN

Colour change is an important event during fruit ripening in blueberry. It is well known that miR156/SPLs act as regulatory modules mediating anthocyanin biosynthesis and ethylene plays critical roles during colour change, but the intrinsic connections between the two pathways remain poorly understood. Previously, we demonstrated that blueberry VcMIR156a/VcSPL12 affects the accumulation of anthocyanins and chlorophylls in tomato and Arabidopsis. In this study, we first showed that VcMIR156a overexpression in blueberry led to enhanced anthocyanin biosynthesis, decreased chlorophyll accumulation, and, intriguingly, concomitant elevation in the expression of ethylene biosynthesis genes and the level of the ethylene precursor ACC. Conversely, VcSPL12 enhanced chlorophyll accumulation and suppressed anthocyanin biosynthesis and ACC synthesis in fruits. Moreover, the treatment with ethylene substitutes and inhibitors attenuated the effects of VcMIR156a and VcSPL12 on pigment accumulation. Protein-DNA interaction assays indicated that VcSPL12 could specifically bind to the promoters and inhibit the activities of the ethylene biosynthetic genes VcACS1 and VcACO6. Collectively, our results show that VcMIR156a/VcSPL12 alters ethylene production through targeting VcACS1 and VcACO6, therefore governing fruit colour change. Additionally, VcSPL12 may directly interact with the promoter region of the chlorophyll biosynthetic gene VcDVR, thereby activating its expression. These findings established an intrinsic connection between the miR156/SPL regulatory module and ethylene pathway.

Asunto(s)

Arabidopsis; Arándanos Azules (Planta); MicroARNs; Frutas/genética; Frutas/metabolismo; Antocianinas; Arándanos Azules (Planta)/genética; Arándanos Azules (Planta)/metabolismo; Color; Proteínas de Plantas/genética; Proteínas de Plantas/metabolismo; Etilenos/metabolismo; Arabidopsis/genética; Clorofila/metabolismo; Regulación de la Expresión Génica de las Plantas/genética; MicroARNs/genética; MicroARNs/metabolismo

Palabras clave

VcMIR156a; VcSPL12; anthocyanins; blueberry; chlorophylls; ethylene

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Arándanos Azules (Planta) / MicroARNs Idioma: En Revista: Plant Biotechnol J Asunto de la revista: BIOTECNOLOGIA / BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido

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