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Physiological ER stress caused by amylase production induces regulated Ire1-dependent mRNA decay in Aspergillus oryzae.
Tanaka, Mizuki; Zhang, Silai; Sato, Shun; Yokota, Jun-Ichi; Sugiyama, Yuko; Kawarasaki, Yasuaki; Yamagata, Youhei; Gomi, Katsuya; Shintani, Takahiro.
Afiliación
  • Tanaka M; Department of Applied Biological Chemistry, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, 183-8509, Japan. mizuki-tanaka@go.tuat.ac.jp.
  • Zhang S; Department of Agricultural Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, 980-8572, Japan.
  • Sato S; Department of Agricultural Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, 980-8572, Japan.
  • Yokota JI; Department of Agricultural Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, 980-8572, Japan.
  • Sugiyama Y; Department of Agricultural Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, 980-8572, Japan.
  • Kawarasaki Y; Biomolecular Engineering Laboratory, School of Food and Nutritional Science, University of Shizuoka, Shizuoka, 422-8526, Japan.
  • Yamagata Y; Department of Applied Biological Chemistry, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, 183-8509, Japan.
  • Gomi K; Department of Agricultural Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, 980-8572, Japan. katsuya.gomi.a6@tohoku.ac.jp.
  • Shintani T; Laboratory of Fermentation Microbiology, Department of Agricultural Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, 980-8572, Japan. katsuya.gomi.a6@tohoku.ac.jp.
Commun Biol ; 6(1): 1009, 2023 10 04.
Article en En | MEDLINE | ID: mdl-37794162
Regulated Ire1-dependent decay (RIDD) is a feedback mechanism in which the endoribonuclease Ire1 cleaves endoplasmic reticulum (ER)-localized mRNAs encoding secretory and membrane proteins in eukaryotic cells under ER stress. RIDD is artificially induced by chemicals that generate ER stress; however, its importance under physiological conditions remains unclear. Here, we demonstrate the occurrence of RIDD in filamentous fungus using Aspergillus oryzae as a model, which secretes copious amounts of amylases. α-Amylase mRNA was rapidly degraded by IreA, an Ire1 ortholog, depending on its ER-associated translation when mycelia were treated with dithiothreitol, an ER-stress inducer. The mRNA encoding maltose permease MalP, a prerequisite for the induction of amylolytic genes, was also identified as an RIDD target. Importantly, RIDD of malP mRNA is triggered by inducing amylase production without any artificial ER stress inducer. Our data provide the evidence that RIDD occurs in eukaryotic microorganisms under physiological ER stress.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aspergillus oryzae / Amilasas Idioma: En Revista: Commun Biol Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aspergillus oryzae / Amilasas Idioma: En Revista: Commun Biol Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido