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Pralidoxime-like reactivator with increased lipophilicity - Molecular modeling and in vitro study.
Kuca, Kamil; Valle da Silva, Jorge Alberto; Nepovimova, Eugenie; Pham, Ngoc Lam; Wu, Wenda; Valis, Martin; Wu, Qinghua; França, Tanos Celmar Costa.
Afiliación
  • Kuca K; Department of Chemistry, Faculty of Science, University of Hradec Kralove, Hradec Kralove, Czech Republic; Biomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic. Electronic address: kamil.kuca@uhk.cz.
  • Valle da Silva JA; Laboratory of Molecular Modeling Applied to the Chemical and Biological Defense (LMCBD), Military Institute of Engineering, Rio de Janeiro/RJ, Brazil.
  • Nepovimova E; Department of Chemistry, Faculty of Science, University of Hradec Kralove, Hradec Kralove, Czech Republic.
  • Pham NL; Department of Chemistry, Faculty of Science, University of Hradec Kralove, Hradec Kralove, Czech Republic.
  • Wu W; Department of Chemistry, Faculty of Science, University of Hradec Kralove, Hradec Kralove, Czech Republic; School of Food and Biological Engineering, Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, China.
  • Valis M; Department of Neurology, University Hospital Hradec Kralove, Hradec Kralove, 500 05, Czech Republic.
  • Wu Q; Department of Chemistry, Faculty of Science, University of Hradec Kralove, Hradec Kralove, Czech Republic; College of Life Science, Yangtze University, Jingzhou, Hubei, China.
  • França TCC; Department of Chemistry, Faculty of Science, University of Hradec Kralove, Hradec Kralove, Czech Republic; Biomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic. Electronic address: tanosfranca@gmail.com.
Chem Biol Interact ; 385: 110734, 2023 Nov 01.
Article en En | MEDLINE | ID: mdl-37788753
Acetylcholinesterase (AChE, EC 3.1.1.7) reactivators (2-PAM, trimedoxime, obidoxime, asoxime) have become an integral part of antidotal treatment in cases of nerve agent and organophosphorus (OP) pesticide poisonings. They are often referred to as specific antidotes due to their ability to restore AChE function when it has been covalently inhibited by an OP compound. Currently available commercial reactivators exhibit limited ability to penetrate the blood-brain barrier, where reactivation of inhibited AChE is crucial. Consequently, there have been numerous efforts to discover more brain-penetrating AChE reactivators. In this study, we examined a derivative of 2-PAM designed to possess increased lipophilicity. This enhanced lipophilicity was achieved through the incorporation of a benzyl group into its molecular structure. Initially, a molecular modeling study was conducted, followed by a comparison of its reactivation efficacy with that of 2-PAM against 10 different AChE inhibitors in vitro. Unfortunately, this relatively significant structural modification of 2-PAM resulted in a decrease in its reactivation potency. Consequently, this derivative cannot be considered as a broad-spectrum AChE reactivator.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reactivadores de la Colinesterasa / Intoxicación por Organofosfatos Límite: Humans Idioma: En Revista: Chem Biol Interact Año: 2023 Tipo del documento: Article Pais de publicación: Irlanda

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reactivadores de la Colinesterasa / Intoxicación por Organofosfatos Límite: Humans Idioma: En Revista: Chem Biol Interact Año: 2023 Tipo del documento: Article Pais de publicación: Irlanda