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A smartphone-assisted colorimetric aptasensor based on aptamer and gold nanoparticles for visual, fast and sensitive detection of ZEN in maize.
Zhang, Liyuan; Chen, Jiayu; Lu, Lifeng; Yu, Runzhong; Zhang, Dongjie.
Afiliación
  • Zhang L; College of Food Science, Heilongjiang Bayi Agricultural University, 5 Xinfeng Road, Daqing 163319, PR China.
  • Chen J; College of Food Science, Heilongjiang Bayi Agricultural University, 5 Xinfeng Road, Daqing 163319, PR China.
  • Lu L; College of Food Science, Heilongjiang Bayi Agricultural University, 5 Xinfeng Road, Daqing 163319, PR China.
  • Yu R; College of Information and Electrical Engineering, Heilongjiang Bayi Agricultural University, 5 Xinfeng Road, Daqing 163319, PR China.
  • Zhang D; College of Food Science, Heilongjiang Bayi Agricultural University, 5 Xinfeng Road, Daqing 163319, PR China.
Food Chem X ; 19: 100792, 2023 Oct 30.
Article en En | MEDLINE | ID: mdl-37780345
A simple, fast, low cost, sensitive, intuitive, visual, label-free, and smartphone-assisted aptamer sensor based on colorimetric assay for the measurement of zearalenone was constructed. The nucleic acid aptamer of zearalenone was used as the recognition element and gold nanoparticles were used as the indicator. Several factors that could influence sensitivity, including the concentration of aptamer and NaCl, and incubation time, and specificity, have been investigated. The results showed that under the optimal conditions, the signal had a good linear relationship when zearalenone concentration is 5-300 ng/mL. A linear regression equation is Y = 0.0003X + 0.5128 (R2 = 0.9989) and a limit of detection is 5 ng/mL. The specificity of the sensor was good. Zearalenone in maize samples were successfully measured. The recoveries of Zearalenone are 81.3 %-96.4 %. The whole process takes only 15 min to complete. The smartphone assisted colorimetric aptamer sensor can be used for the detection of zearalenone in maize.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Food Chem X Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Food Chem X Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos