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l-fucoside localization in the gills of the genus Takifugu and its possible implication in the parasitism of Heterobothrium okamotoi (Monogenea: Diclidophoridae).
Sato, Yoshiki; Matsunaga, Ryohei; Tasumi, Satoshi; Mizuno, Naoki; Nakane, Motoyuki; Hosoya, Sho; Yamamoto, Atsushi; Nakamura, Osamu; Tsutsui, Shigeyuki; Shiozaki, Kazuhiro; Kikuchi, Kiyoshi.
Afiliación
  • Sato Y; Fisheries Laboratory, Graduate School of Agricultural and Life sciences, The University of Tokyo, 2971-4 Bentenjima, Maisaka, Nishi-ku, Hamamatsu, Shizuoka 431-0214, Japan.
  • Matsunaga R; Fisheries Laboratory, Graduate School of Agricultural and Life sciences, The University of Tokyo, 2971-4 Bentenjima, Maisaka, Nishi-ku, Hamamatsu, Shizuoka 431-0214, Japan.
  • Tasumi S; The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima 890-0065, Japan. Electronic address: tasumi@fish.kagoshima-u.ac.jp.
  • Mizuno N; Fisheries Laboratory, Graduate School of Agricultural and Life sciences, The University of Tokyo, 2971-4 Bentenjima, Maisaka, Nishi-ku, Hamamatsu, Shizuoka 431-0214, Japan.
  • Nakane M; Kumamoto Prefectural Fisheries Research Center, 2450-2 Oyanomachinaka, Amakusa, Kumamoto 869-3603, Japan.
  • Hosoya S; Fisheries Laboratory, Graduate School of Agricultural and Life sciences, The University of Tokyo, 2971-4 Bentenjima, Maisaka, Nishi-ku, Hamamatsu, Shizuoka 431-0214, Japan.
  • Yamamoto A; The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima 890-0065, Japan.
  • Nakamura O; School of Marine Biosciences, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan.
  • Tsutsui S; School of Marine Biosciences, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan.
  • Shiozaki K; The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima 890-0065, Japan.
  • Kikuchi K; Fisheries Laboratory, Graduate School of Agricultural and Life sciences, The University of Tokyo, 2971-4 Bentenjima, Maisaka, Nishi-ku, Hamamatsu, Shizuoka 431-0214, Japan.
Biochim Biophys Acta Gen Subj ; 1867(12): 130467, 2023 12.
Article en En | MEDLINE | ID: mdl-37777092
BACKGROUND: The monogenean parasite Heterobothrium okamotoi only parasitizes the gills of Takifugu rubripes. In this study, we hypothesized that the carbohydrates contribute to high host specificity of H. okamotoi. METHODS: T. rubripes, T. niphobles, T. snyderi, and T. pardalis were used for UEA I staining of the gills and an in vivo challenge test against H. okamotoi. To examine the effect of l-fucose, an in vitro detachment test was conducted using the host's gills. Additionally, fucosylated proteins were isolated from the membrane proteins of T. niphobles gills. RESULTS: The location of l-fucoside and the infection dynamics in four species were correlated to some extent; H. okamotoi detached relatively quickly from T. niphobles possessing l-fucoside both on the surface of the gills and in certain types of cells, including mucus cells, but detached slowly from T. snyderi possessing l-fucoside in only certain types of cells, including mucus cells. Under the conditions examined, H. okamotoi exhibited minimal detachment from T. rubripes and T. pardalis, and l-fucoside was not detected. The significantly higher detachment rate of H. okamotoi from the host's gills incubated in l-fucose-containing medium compared with the controls suggests that l-fucose in the non-host gills induced detachment of H. okamotoi. Four fucosylated proteins, including mucin5AC-like, were identified as potential factors for the detachment of H. okamotoi. CONCLUSIONS: Fucosylated proteins covering the surface of non-host gills might contribute to H. okamotoi detachment. GENERAL SIGNIFICANCE: This research shows the possible involvement of oligosaccharides in the host specificity of monogenean parasites.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trematodos / Infecciones por Trematodos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Biochim Biophys Acta Gen Subj Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trematodos / Infecciones por Trematodos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Biochim Biophys Acta Gen Subj Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Países Bajos