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Segmented, Negative-Sense RNA Viruses of Humans: Genetic Systems and Experimental Uses of Reporter Strains.
Hamele, Cait E; Spurrier, M Ariel; Leonard, Rebecca A; Heaton, Nicholas S.
Afiliación
  • Hamele CE; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, North Carolina, USA; email: nicholas.heaton@duke.edu.
  • Spurrier MA; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, North Carolina, USA; email: nicholas.heaton@duke.edu.
  • Leonard RA; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, North Carolina, USA; email: nicholas.heaton@duke.edu.
  • Heaton NS; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, North Carolina, USA; email: nicholas.heaton@duke.edu.
Annu Rev Virol ; 10(1): 261-282, 2023 09 29.
Article en En | MEDLINE | ID: mdl-37774125
Negative-stranded RNA viruses are a large group of viruses that encode their genomes in RNA across multiple segments in an orientation antisense to messenger RNA. Their members infect broad ranges of hosts, and there are a number of notable human pathogens. Here, we examine the development of reverse genetic systems as applied to these virus families, emphasizing conserved approaches illustrated by some of the prominent members that cause significant human disease. We also describe the utility of their genetic systems in the development of reporter strains of the viruses and some biological insights made possible by their use. To conclude the review, we highlight some possible future uses of reporter viruses that not only will increase our basic understanding of how these viruses replicate and cause disease but also could inform the development of new approaches to therapeutically intervene.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus ARN / Virus ARN de Sentido Negativo Límite: Humans Idioma: En Revista: Annu Rev Virol Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus ARN / Virus ARN de Sentido Negativo Límite: Humans Idioma: En Revista: Annu Rev Virol Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos