MicroRNA-25/93 induction by Vpu as a mechanism for counteracting MARCH1-restriction on HIV-1 infectivity in macrophages.

Lodge, Robert; Xu, Zaikun; Eklund, Mckenna; Stürzel, Christina; Kirchhoff, Frank; Tremblay, Michel J; Hobman, Tom C; Cohen, Éric A

Lodge, Robert; Xu, Zaikun; Eklund, Mckenna; Stürzel, Christina; Kirchhoff, Frank; Tremblay, Michel J; Hobman, Tom C; Cohen, Éric A.

Afiliación

Lodge R; Laboratory of Human Retrovirology, Institut de recherches cliniques de Montréal (IRCM) , Montreal, Quebec, Canada.
Xu Z; Department of Cell Biology, University of Alberta , Edmonton, Alberta, Canada.
Eklund M; Department of Cell Biology, University of Alberta , Edmonton, Alberta, Canada.
Stürzel C; Institute of Molecular Virology, Ulm University Medical Center , Ulm, Germany.
Kirchhoff F; Institute of Molecular Virology, Ulm University Medical Center , Ulm, Germany.
Tremblay MJ; Centre de recherche du centre hospitalier universitaire de Québec, Université Laval , Quebec City, Quebec, Canada.
Hobman TC; Département de microbiologie-infectiologie et immunologie, Faculté de médecine, Université Laval , Quebec City, Quebec, Canada.
Cohen ÉA; Department of Cell Biology, University of Alberta , Edmonton, Alberta, Canada.

mBio ; 14(5): e0195023, 2023 Oct 31.

Article en En | MEDLINE | ID: mdl-37773002

RESUMEN

IMPORTANCE: In order to efficiently produce infectious viral particles, HIV must counter several restrictions exerted by host cell antiviral proteins. MARCH1 is a member of the MARCH protein family that restricts HIV infection by limiting the incorporation of viral envelope glycoproteins into nascent virions. Here, we identified two regulatory RNAs, microRNAs-25 and -93, induced by the HIV-1 accessory protein Vpu, that downregulate MARCH1 mRNA. We also show that Vpu induces these cellular microRNAs in macrophages by hijacking the cellular ß-catenin pathway. The notion that HIV-1 has evolved a mechanism to counteract MARCH1 restriction on viral infectivity underlines the importance of MARCH1 in the host antiviral response.

Asunto(s)

Infecciones por VIH; Seropositividad para VIH; VIH-1; MicroARNs; Humanos; Infecciones por VIH/metabolismo; VIH-1/fisiología; Proteínas Reguladoras y Accesorias Virales/genética; Proteínas Reguladoras y Accesorias Virales/metabolismo; Proteínas del Virus de la Inmunodeficiencia Humana/genética; Antivirales/metabolismo; MicroARNs/genética; MicroARNs/metabolismo; Macrófagos/metabolismo; Proteínas Ligadas a GPI/metabolismo

Palabras clave

HIV-1 countermeasures; MARCH proteins; Vpu; beta-catenin; human immunodeficiency virus; macrophages; microRNA; restriction factor; type-I interferon response; viral glycoproteins

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por VIH / VIH-1 / Seropositividad para VIH / MicroARNs Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: MBio Año: 2023 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos

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