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Liquid-liquid phase separation within fibrillar networks.
Liu, Jason X; Haataja, Mikko P; Kosmrlj, Andrej; Datta, Sujit S; Arnold, Craig B; Priestley, Rodney D.
Afiliación
  • Liu JX; Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ, 08544, USA.
  • Haataja MP; Princeton Materials Institute, Princeton University, Princeton, NJ, 08544, USA.
  • Kosmrlj A; Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ, 08544, USA.
  • Datta SS; Princeton Materials Institute, Princeton University, Princeton, NJ, 08544, USA.
  • Arnold CB; Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ, 08544, USA.
  • Priestley RD; Princeton Materials Institute, Princeton University, Princeton, NJ, 08544, USA.
Nat Commun ; 14(1): 6085, 2023 09 29.
Article en En | MEDLINE | ID: mdl-37770446
Complex fibrillar networks mediate liquid-liquid phase separation of biomolecular condensates within the cell. Mechanical interactions between these condensates and the surrounding networks are increasingly implicated in the physiology of the condensates and yet, the physical principles underlying phase separation within intracellular media remain poorly understood. Here, we elucidate the dynamics and mechanics of liquid-liquid phase separation within fibrillar networks by condensing oil droplets within biopolymer gels. We find that condensates constrained within the network pore space grow in abrupt temporal bursts. The subsequent restructuring of condensates and concomitant network deformation is contingent on the fracture of network fibrils, which is determined by a competition between condensate capillarity and network strength. As a synthetic analog to intracellular phase separation, these results further our understanding of the mechanical interactions between biomolecular condensates and fibrillar networks in the cell.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Citoesqueleto / Fracturas Óseas Límite: Humans Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Citoesqueleto / Fracturas Óseas Límite: Humans Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido