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Activation of Wnt Pathway Suppresses Growth of MUG-Chor1 Chordoma Cell Line.
Sisli, Hatice Burcu; Hayal, Taha Bartu; Senkal, Selinay; Bulut, Ezgi; Kiratli, Binnur; Asutay, Ayla Burçin; Sahin, Fikrettin; Bayrak, Ömer Faruk; Dogan, Aysegül.
Afiliación
  • Sisli HB; Faculty of Engineering, Genetics and Bioengineering Department, Yeditepe University, Istanbul, 34755, Turkey.
  • Hayal TB; Faculty of Engineering, Genetics and Bioengineering Department, Yeditepe University, Istanbul, 34755, Turkey.
  • Senkal S; Faculty of Engineering, Genetics and Bioengineering Department, Yeditepe University, Istanbul, 34755, Turkey.
  • Bulut E; Faculty of Engineering, Genetics and Bioengineering Department, Yeditepe University, Istanbul, 34755, Turkey.
  • Kiratli B; Faculty of Engineering, Genetics and Bioengineering Department, Yeditepe University, Istanbul, 34755, Turkey.
  • Asutay AB; Faculty of Engineering, Genetics and Bioengineering Department, Yeditepe University, Istanbul, 34755, Turkey.
  • Sahin F; Faculty of Engineering, Genetics and Bioengineering Department, Yeditepe University, Istanbul, 34755, Turkey.
  • Bayrak ÖF; Department of Medical Genetics, School of Medicine, Yeditepe University, Istanbul, 34755, Turkey.
  • Dogan A; Faculty of Engineering, Genetics and Bioengineering Department, Yeditepe University, Istanbul, 34755, Turkey. aysegul.dogan@yeditepe.edu.tr.
Cell Biochem Biophys ; 81(4): 823-837, 2023 Dec.
Article en En | MEDLINE | ID: mdl-37751039
Chordoma as a malignant bone tumor, occurs along the axial skeleton and does not have an effective therapy. Brachyury, which is a crucial player for the formation of early embryonic notochord, is abundantly found in both sporadic and familial chordoma. During embryonic development, Brachyury expression was reported to be regulated by the Wnt pathway. The objective of the study is to investigate the role of Wnt signaling in a human chordoma cell line in terms of proliferation, survival, and invasiveness. We tried to elucidate the signaling events that regulate Chordoma cancer. In this regard, Wnt pathway was activated or inhibited using various strategies including small molecules, siRNA-based knockdown and overexpression applications. The results indicated the negative regulatory effect of Wnt signaling activity on proliferation and migration capacity of the chordoma cells. It was revealed that when GSK3ß was inhibited, the Wnt pathway was activated and negatively regulated T/Bra expression. Activity of the Wnt pathway caused cell cycle arrest, reduced migration potential of the cells, and led to cell death. Therefore, the present study suggests that the Wnt pathway plays a key role in suppressing the proliferation and invasive characteristics of human chordoma cells and has a great potential as a therapeutic target in further clinical studies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cordoma / Vía de Señalización Wnt Límite: Humans Idioma: En Revista: Cell Biochem Biophys Asunto de la revista: BIOFISICA / BIOQUIMICA Año: 2023 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Estados Unidos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cordoma / Vía de Señalización Wnt Límite: Humans Idioma: En Revista: Cell Biochem Biophys Asunto de la revista: BIOFISICA / BIOQUIMICA Año: 2023 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Estados Unidos