Histological and Immunohistochemical Investigation of the Cerebellum in Porcupine and Guinea Pig.
Microsc Microanal
; 29(3): 1289-1297, 2023 06 09.
Article
en En
| MEDLINE
| ID: mdl-37749681
The present study was designed to investigate the cerebellum histology and immunohistochemistry in porcupine (Hystrix cristata) and guinea pig (Cavia porcellus). Two adult porcupines and two adult guinea pigs were used. For general histology, crystal violet and Luxol fast blue stains were applied. For immunohistochemistry, myelin-associated glycoprotein (MAG), neurofilament 200 (NF200), calbindin D-28K, and glial fibrillary-associated protein (GFAP) were investigated. The cerebellar cortex in both species was composed of three cellular layers: molecular, granular, and Purkinje cell (PC) layers. Purkinje cells in the porcupine showed a purple-colored and dark blue-colored cytoplasm in reaction to the crystal violet and Luxol fast blue staining, respectively. In the guinea pig, PC has a uniform reaction to the Luxol fast blue with dark-blue-colored cytoplasm. However, in response to the crystal violet, some PC with dark-purple cytoplasm showed stronger reaction than other PC which showed light-purple cytoplasm. The PC layer in some folia of the porcupine cerebellum was composed of 2-3 layers. The expression rates of calbindin D-28K, MAG, GFAP, and NF200 in the porcupine cerebellum were determined to be 19%, 42.5%, 62%, and 30%, respectively. These values were determined to be 27%, 34%, 43.5%, and 31.5%, respectively, in the guinea pig cerebellum.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Puercoespines
Límite:
Animals
Idioma:
En
Revista:
Microsc Microanal
Año:
2023
Tipo del documento:
Article
País de afiliación:
Irán
Pais de publicación:
Reino Unido