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Development of a specific MPXV antigen detection immunodiagnostic assay.
Davis, Ian; Payne, Jackie M; Olguin, Victoria L; Sanders, Madison P; Clements, Tamara; Stefan, Christopher P; Williams, Janice A; Hooper, Jay W; Huggins, John W; Mucker, Eric M; Ricks, Keersten M.
Afiliación
  • Davis I; Diagnostic Systems Division, United States Army Medical Research Institute of Diseases, Frederick, MD, United States.
  • Payne JM; Diagnostic Systems Division, United States Army Medical Research Institute of Diseases, Frederick, MD, United States.
  • Olguin VL; Diagnostic Systems Division, United States Army Medical Research Institute of Diseases, Frederick, MD, United States.
  • Sanders MP; Diagnostic Systems Division, United States Army Medical Research Institute of Diseases, Frederick, MD, United States.
  • Clements T; Diagnostic Systems Division, United States Army Medical Research Institute of Diseases, Frederick, MD, United States.
  • Stefan CP; Diagnostic Systems Division, United States Army Medical Research Institute of Diseases, Frederick, MD, United States.
  • Williams JA; Pathology Division, United States Army Medical Research Institute of Diseases, Frederick, MD, United States.
  • Hooper JW; Virology Division, United States Army Medical Research Institute of Diseases, Frederick, MD, United States.
  • Huggins JW; Virology Division, United States Army Medical Research Institute of Diseases, Frederick, MD, United States.
  • Mucker EM; Virology Division, United States Army Medical Research Institute of Diseases, Frederick, MD, United States.
  • Ricks KM; Diagnostic Systems Division, United States Army Medical Research Institute of Diseases, Frederick, MD, United States.
Front Microbiol ; 14: 1243523, 2023.
Article en En | MEDLINE | ID: mdl-37744911
Human monkeypox (mpox) has recently become a global public health emergency; however, assays that detect mpox infection are not widely available, largely due to cross-reactivity within the Orthopoxvirus genus. Immunoassay development was largely confined to researchers who focus on biothreats and endemic areas (Central and West Africa) until the 2022 outbreak. As was noted in the COVID-19 pandemic, antigen detection assays, integrated with molecular assays, are necessary to help curb the spread of disease. Antigen-detecting immunoassays offer the advantage of providing results ranging from within min to h and in lateral flow formats; they can be deployed for point-of-care, home, or field use. This study reports the development of an mpox-specific antigen detection immunoassay developed on a multiplexed, magnetic-bead-based platform utilizing reagents from all research sectors (commercial, academic, and governmental). Two semi-quantitative assays were developed in parallel and standardized with infectious mpox virus (MPXV) cell culture fluid and MPXV-positive non-human primate (NHP) sera samples. These assays could detect viral antigens in serum, were highly specific toward MPXV as compared to other infectious orthopoxviruses (vaccinia virus, cowpox virus, and camelpox virus), and exhibited a correlation with quantitative PCR results from an NHP study. Access to a toolbox of assays for mpox detection will be key for identifying cases and ensuring proper treatment, as MPXV is currently a global traveler.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Front Microbiol Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Front Microbiol Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza