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Chitosan-gentamicin conjugate attenuates heat stress-induced intestinal barrier injury via the TLR4/STAT6/MYLK signaling pathway: In vitro and in vivo studies.
Niu, Xueting; Hu, Canying; Chen, Shengwei; Wen, Jiaying; Liu, Xiaoxi; Yong, Yanhong; Yu, Zhichao; Ma, Xingbin; Li, Chengpeng; Warda, Mohamad; Abd El-Aty, A M; Gooneratne, Ravi; Ju, Xianghong.
Afiliación
  • Niu X; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China; Marine Medical Research and Development Centre, Shenzheng Institute of Guangdong Ocean University, Shenzheng 518120, China.
  • Hu C; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China; Marine Medical Research and Development Centre, Shenzheng Institute of Guangdong Ocean University, Shenzheng 518120, China.
  • Chen S; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China; Marine Medical Research and Development Centre, Shenzheng Institute of Guangdong Ocean University, Shenzheng 518120, China.
  • Wen J; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China; Marine Medical Research and Development Centre, Shenzheng Institute of Guangdong Ocean University, Shenzheng 518120, China.
  • Liu X; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.
  • Yong Y; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.
  • Yu Z; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.
  • Ma X; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.
  • Li C; College of Chemistry and Environment Science, Guangdong Ocean University, Zhanjiang 524088, China.
  • Warda M; Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
  • Abd El-Aty AM; Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt; Department of Medical Pharmacology, Medical Faculty, Ataturk University, Erzurum 25240, Turkey.
  • Gooneratne R; Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand.
  • Ju X; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China; Marine Medical Research and Development Centre, Shenzheng Institute of Guangdong Ocean University, Shenzheng 518120, China. Electronic address: juxh77@163.com.
Carbohydr Polym ; 321: 121279, 2023 Dec 01.
Article en En | MEDLINE | ID: mdl-37739521
Heat stress (HS) has a negative impact on animal health. A modified chitosan-gentamicin conjugate (CS-GT) was prepared to investigate its potential protective effects and mechanism of action on heat stress-induced intestinal mucosa injury in IPEC-J2 cells and mouse 3D intestinal organs in a mouse model. CS-GT significantly (P < 0.01) reversed the decline in transmembrane resistance and increased the FITC-dextran permeability of the IPEC-J2 monolayer fusion epithelium caused by heat stress. Heat stress decreased the expression of the tight binding proteins occludin, claudin1, and claudin2. However, pretreatment with CS-GT significantly increased (P < 0.01) the expression of these tight binding proteins. Mechanistically, CS-GT inhibited the activation of the TLR4/STAT6/MYLK signaling pathway induced by heat stress. Molecular docking showed that CS-GT can bind effectively with TLR4. In conclusion, CS-GT alleviates heat stress-induced intestinal mucosal damage both in vitro and in vivo. This effect is mediated, at least partly, by the inhibition of the TLR4/STAT6/MYLK signaling pathway and upregulation of tight junction proteins. These findings suggest that CS-GT may have therapeutic potential in the prevention and treatment of heat stress-related intestinal injury.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quemaduras / Quitosano Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Carbohydr Polym Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quemaduras / Quitosano Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Carbohydr Polym Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido