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Preliminary study of chromosome aberrations using Giemsa, two-colour fish, and micronucleus assays in lymphocytes of individuals living in elevated radon concentration areas.
Ramadhani, Dwi; Purnami, Sofiati; Suvifan, Viria Agesti; Wanandi, Septelia Inawati; Wibowo, Heri; Syaifudin, Mukh.
Afiliación
  • Ramadhani D; Doctoral Program for Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 6, DKI Jakarta, 10430, Indonesia.
  • Purnami S; Research Center for Radioisotope, Radiopharmaceutical and Biodosimetry Technology, Research Organization for Nuclear Energy, National Research and Innovation Agency, Jl. Kw. Puspitek Blok NN No. 11, Muncul, Setu, Tangerang, 15314, Indonesia.
  • Suvifan VA; Research Center for Safety, Metrology and Nuclear Quality Technology, Research Organization for Nuclear Energy, National Research and Innovation Agency, Jl. Lebak Bulus Raya No. 49, DKI Jakarta, 12440, Indonesia.
  • Wanandi SI; Research Center for Safety, Metrology and Nuclear Quality Technology, Research Organization for Nuclear Energy, National Research and Innovation Agency, Jl. Lebak Bulus Raya No. 49, DKI Jakarta, 12440, Indonesia.
  • Wibowo H; Department of Biochemistry & Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 6, DKI Jakarta, 10430, Indonesia.
  • Syaifudin M; Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 6, DKI Jakarta, 10430, Indonesia.
Radiat Prot Dosimetry ; 199(14): 1508-1515, 2023 Sep 18.
Article en En | MEDLINE | ID: mdl-37721082
The frequencies of unstable and stable chromosome aberrations and micronuclei were examined in peripheral blood samples from 10 individuals living in elevated radon concentration areas (Takandeang Village, Mamuju, Indonesia). Blood samples from 10 people living in Topoyo Village were used as a control group. For unstable chromosome aberration analysis, a dicentric chromosome assay was conducted using conventional Giemsa staining. Chromosomal painting of chromosomes 1 and 4 using the fluorescence in situ hybridisation technique was also applied to four subjects to assess the stable chromosome aberration. Our study showed no significant increases across all groups in dicentric and other unstable chromosome aberrations, such as rings and acentric fragments. Translocations were found in one person from Takandeang Village and two Topoyo Village inhabitants. The translocations found in the subjects from Takandeang Village were due more to aging factors than to radon exposure. The number of micronuclei per 1000 binucleus cells in Takandeang Village inhabitants was not significantly different than that in the control group (p = 0.943). A more comprehensive analysis should be conducted in a subsequent study by increasing the number of study donors and the number of metaphases to be analysed in both dicentric chromosome assay and fluorescence in situ hybridisation assays. Such research could provide valid information on the cytogenetic effects of elevated indoor radon exposure.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aberraciones Cromosómicas / Radón Límite: Animals Idioma: En Revista: Radiat Prot Dosimetry Año: 2023 Tipo del documento: Article País de afiliación: Indonesia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aberraciones Cromosómicas / Radón Límite: Animals Idioma: En Revista: Radiat Prot Dosimetry Año: 2023 Tipo del documento: Article País de afiliación: Indonesia Pais de publicación: Reino Unido