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Assessing the capacity of methylated DNA markers of cervical squamous cell carcinoma to discriminate oropharyngeal squamous cell carcinoma in human papillomavirus mediated disease.
Bartemes, Kathleen R; Gochanour, Benjamin R; Routman, David M; Ma, Daniel J; Doering, Karen A; Burger, Kelli N; Foote, Patrick H; Taylor, William R; Mahoney, Douglas W; Berger, Calise K; Cao, Xiaoming; Then, Sara S; Haller, Travis J; Larish, Alyssa M; Moore, Eric J; Garcia, Joaquin J; Graham, Rondell P; Bakkum-Gamez, Jamie N; Kisiel, John B; Van Abel, Kathryn M.
Afiliación
  • Bartemes KR; Department of Otolaryngology, Head and Neck Surgery, Mayo Clinic, Rochester, MN, USA.
  • Gochanour BR; Department of Quantitative Health Sciences, Mayo Clinic, Rochester, MN, USA.
  • Routman DM; Department of Radiation Oncology, Rochester, MN, USA.
  • Ma DJ; Department of Radiation Oncology, Rochester, MN, USA.
  • Doering KA; Department of Gastroenterology, Rochester, MN, USA.
  • Burger KN; Department of Quantitative Health Sciences, Mayo Clinic, Rochester, MN, USA.
  • Foote PH; Department of Gastroenterology, Rochester, MN, USA.
  • Taylor WR; Department of Gastroenterology, Rochester, MN, USA.
  • Mahoney DW; Department of Quantitative Health Sciences, Mayo Clinic, Rochester, MN, USA.
  • Berger CK; Department of Gastroenterology, Rochester, MN, USA.
  • Cao X; Department of Gastroenterology, Rochester, MN, USA.
  • Then SS; Department of Gastroenterology, Rochester, MN, USA.
  • Haller TJ; Department of Otolaryngology, Head and Neck Surgery, Mayo Clinic, Rochester, MN, USA.
  • Larish AM; Department of Obstetrics and Gynecology, Mayo Clinic, Rochester, MN, USA.
  • Moore EJ; Department of Otolaryngology, Head and Neck Surgery, Mayo Clinic, Rochester, MN, USA.
  • Garcia JJ; Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.
  • Graham RP; Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.
  • Bakkum-Gamez JN; Department of Obstetrics and Gynecology, Mayo Clinic, Rochester, MN, USA.
  • Kisiel JB; Department of Gastroenterology, Rochester, MN, USA.
  • Van Abel KM; Department of Otolaryngology, Head and Neck Surgery, Mayo Clinic, Rochester, MN, USA. Electronic address: vanabel.kathryn@mayo.edu.
Oral Oncol ; 146: 106568, 2023 11.
Article en En | MEDLINE | ID: mdl-37717549
OBJECTIVE: Early identification of human papillomavirus associated oropharyngeal squamous cell carcinoma (HPV(+)OPSCC) is challenging and novel biomarkers are needed. We hypothesized that a panel of methylated DNA markers (MDMs) found in HPV(+) cervical squamous cell carcinoma (CSCC) will have similar discrimination in HPV(+)OPSCC tissues. MATERIALS AND METHODS: Formalin-fixed, paraffin-embedded tissues were obtained from patients with primary HPV(+)OPSCC or HPV(+)CSCC; control tissues included normal oropharynx palatine tonsil (NOP) and cervix (NCS). Using a methylation-specific polymerase chain reaction, 21 previously validated cervical MDMs were evaluated on tissue-extracted DNA. Discrimination between case and control cervical and oropharynx tissue was assessed using area under the curve (AUC). RESULTS: 34 HPV(+)OPSCC, 36 HPV(+)CSCC, 26 NOP, and 24 NCS patients met inclusion criteria. Within HPV(+)CSCC, 18/21 (86%) of MDMs achieved an AUC ≥ 0.9 and all MDMs exhibited better than chance classifications relative to control cervical tissue (all p < 0.001). In contrast, within HPV(+)OPSCC only 5/21 (24%) MDMs achieved an AUC ≥ 0.90 but 19/21 (90%) exhibited better than chance classifications relative to control tonsil tissue (all p < 0.001). Overall, 13/21 MDMs had statistically significant lower AUCs in the oropharyngeal cohort compared to the cervical cohort, and only 1 MDM exhibited a statistically significant increase in AUC. CONCLUSIONS: Previously validated MDMs exhibited robust performance in independent HPV(+)CSCC patients. However, most of these MDMs exhibited higher discrimination for HPV(+)CSCC than for HPV(+)OPSCC. This suggests that each SCC subtype requires a unique set of MDMs for optimal discrimination. Future studies are necessary to establish an MDM panel for HPV(+)OPSCC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma de Células Escamosas / Neoplasias Orofaríngeas / Neoplasias del Cuello Uterino / Infecciones por Papillomavirus / Neoplasias de Cabeza y Cuello Tipo de estudio: Prognostic_studies Límite: Female / Humans Idioma: En Revista: Oral Oncol Asunto de la revista: NEOPLASIAS Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma de Células Escamosas / Neoplasias Orofaríngeas / Neoplasias del Cuello Uterino / Infecciones por Papillomavirus / Neoplasias de Cabeza y Cuello Tipo de estudio: Prognostic_studies Límite: Female / Humans Idioma: En Revista: Oral Oncol Asunto de la revista: NEOPLASIAS Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido