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Integrated modeling of the Nexin-dynein regulatory complex reveals its regulatory mechanism.
Ghanaeian, Avrin; Majhi, Sumita; McCafferty, Caitlyn L; Nami, Babak; Black, Corbin S; Yang, Shun Kai; Legal, Thibault; Papoulas, Ophelia; Janowska, Martyna; Valente-Paterno, Melissa; Marcotte, Edward M; Wloga, Dorota; Bui, Khanh Huy.
Afiliación
  • Ghanaeian A; Department of Anatomy and Cell Biology, Faculty of Medicine and Health Sciences, McGill University, Québec, Canada.
  • Majhi S; Laboratory of Cytoskeleton and Cilia Biology, Nencki Institute of Experimental Biology of Polish Academy of Sciences, 3 Pasteur Street, 02-093, Warsaw, Poland.
  • McCafferty CL; Department of Molecular Biosciences, Center for Systems and Synthetic Biology, University of Texas, Austin, TX, USA.
  • Nami B; Genetics and Genome Biology Program, Hospital for Sick Children, Toronto, Canada.
  • Black CS; Department of Anatomy and Cell Biology, Faculty of Medicine and Health Sciences, McGill University, Québec, Canada.
  • Yang SK; Department of Anatomy and Cell Biology, Faculty of Medicine and Health Sciences, McGill University, Québec, Canada.
  • Legal T; Department of Anatomy and Cell Biology, Faculty of Medicine and Health Sciences, McGill University, Québec, Canada.
  • Papoulas O; Department of Molecular Biosciences, Center for Systems and Synthetic Biology, University of Texas, Austin, TX, USA.
  • Janowska M; Laboratory of Cytoskeleton and Cilia Biology, Nencki Institute of Experimental Biology of Polish Academy of Sciences, 3 Pasteur Street, 02-093, Warsaw, Poland.
  • Valente-Paterno M; Laboratory of Immunology, Mossakowski Institute of Experimental and Clinical Medicine, Polish Academy of Science, Pawinskiego 5, 02-106, Warsaw, Poland.
  • Marcotte EM; Department of Anatomy and Cell Biology, Faculty of Medicine and Health Sciences, McGill University, Québec, Canada.
  • Wloga D; Department of Molecular Biosciences, Center for Systems and Synthetic Biology, University of Texas, Austin, TX, USA.
  • Bui KH; Laboratory of Cytoskeleton and Cilia Biology, Nencki Institute of Experimental Biology of Polish Academy of Sciences, 3 Pasteur Street, 02-093, Warsaw, Poland. d.wloga@nencki.edu.pl.
Nat Commun ; 14(1): 5741, 2023 09 15.
Article en En | MEDLINE | ID: mdl-37714832
Cilia are hairlike protrusions that project from the surface of eukaryotic cells and play key roles in cell signaling and motility. Ciliary motility is regulated by the conserved nexin-dynein regulatory complex (N-DRC), which links adjacent doublet microtubules and regulates and coordinates the activity of outer doublet complexes. Despite its critical role in cilia motility, the assembly and molecular basis of the regulatory mechanism are poorly understood. Here, using cryo-electron microscopy in conjunction with biochemical cross-linking and integrative modeling, we localize 12 DRC subunits in the N-DRC structure of Tetrahymena thermophila. We also find that the CCDC96/113 complex is in close contact with the DRC9/10 in the linker region. In addition, we reveal that the N-DRC is associated with a network of coiled-coil proteins that most likely mediates N-DRC regulatory activity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dineínas / Proteínas Asociadas a Microtúbulos Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2023 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dineínas / Proteínas Asociadas a Microtúbulos Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2023 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido