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[Guizhi Fuling Capsule inhibits migration and induces apoptosis of human ovarian cancer cells by regulating the NF-κB signaling pathway].
Guo, X; Chen, L; Lü, Q; DU, R; Luo, Q; Zhang, Y; Bian, H; Han, L.
Afiliación
  • Guo X; Zhang Zhongjing School of Chinese Medicine,Nanyang Institute of Technology, Nanyang 473061, China.
  • Chen L; Zhang Zhongjing School of Chinese Medicine,Nanyang Institute of Technology, Nanyang 473061, China.
  • Lü Q; Henan Key Laboratory of ZHANG Zhongjing Formulae and Herbs for Immunoregulation,Nanyang Institute of Technology, Nanyang 473061, China.
  • DU R; Department of Chinese Medicine, Nanyang Medical College, Nanyang 473061, China.
  • Luo Q; Zhang Zhongjing School of Chinese Medicine,Nanyang Institute of Technology, Nanyang 473061, China.
  • Zhang Y; Henan Key Laboratory of ZHANG Zhongjing Formulae and Herbs for Immunoregulation,Nanyang Institute of Technology, Nanyang 473061, China.
  • Bian H; Zhang Zhongjing School of Chinese Medicine,Nanyang Institute of Technology, Nanyang 473061, China.
  • Han L; Zhang Zhongjing School of Chinese Medicine,Nanyang Institute of Technology, Nanyang 473061, China.
Nan Fang Yi Ke Da Xue Xue Bao ; 43(8): 1315-1321, 2023 Aug 20.
Article en Zh | MEDLINE | ID: mdl-37712267
OBJECTIVE: To study the inhibitory effect of Guizhi Fuling Capsule (GFC) on migration of human ovarian cancer cells and explore the possible mechanism. METHODS: Sixty Wistar rats were randomized into 4 groups for daily gavage of saline or 4, 8, or 16 g/kg GFC suspension for 5 days to prepare blank and low-, medium- and high-dose GFC-medicated sera. Cisplatinresistant ovarian cancer SKOV3/DDP cells were treated with these sera with nuclear factor-κB (NF-κB) inhibitor SN50 as the positive control, and the changes in migration ability and apoptosis of the cells were examined using scratch assay and flow cytometry, respectively; the changes in the mRNA and protein expressions of CDH1, CDH2, caspase 3 and NF- κB were detected using RT-qPCR and Western blotting. ATAC-seq was used to analyze the changes in expressions of CDH1, CDH2, caspase 3 and NF-κB genes in the open chromatin. RESULTS: Treatment with GFC-medicated sera dose-dependently inhibited the migration (P < 0.05), increased apoptosis (P < 0.01), inhibited CDH2 and NF-κB mRNA expression (P < 0.05), and enhanced caspase 3 and CDH1 mRNA expressions (P < 0.01) in SKOV3/DDP cells. The effects of high-dose GFC-medicated serum were comparable to those of SN50 (P>0.05), but its effect for enhancing DH1 protein expression was weaker than that of SN50 (P < 0.01). GFC-medicated sera significantly lowered the expressions of NF-κB and CDH2 and increased CDH1 expression in the open chromatin without obviously affecting caspase 3 expression. CONCLUSION: GFC- medicated sera inhibits the migration ability of SKOV3/DDP cells possibly by promoting cell apoptosis and caspase 3 and CDH1 expressions, inhibiting CDH2 and NF-κB expressions, and regulating the expressions of NF-κB, CDH2 and CDH1 in the open chromatin.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Ováricas / Wolfiporia Tipo de estudio: Clinical_trials Límite: Animals / Female / Humans Idioma: Zh Revista: Nan Fang Yi Ke Da Xue Xue Bao Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: China
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Ováricas / Wolfiporia Tipo de estudio: Clinical_trials Límite: Animals / Female / Humans Idioma: Zh Revista: Nan Fang Yi Ke Da Xue Xue Bao Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: China