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Analysis of the immunostimulatory effects of cytokine-expressing internal ribosome entry site-based RNA adjuvants and their applications.
Lee, Yu-Sun; Bang, Yoo-Jin; Yoo, Soyeon; Park, Sang-In; Park, Hyo-Jung; Kwak, Hye Won; Bae, Seo-Hyeon; Park, Hyeong-Jun; Kim, Jae-Yong; Youn, Sue-Bean; Roh, Gahyun; Lee, Seonghyun; Kwon, Sung Pil; Bang, Eun-Kyoung; Keum, Gyochang; Nam, Jae-Hwan; Hong, So-Hee.
Afiliación
  • Lee YS; Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do, Republic of Korea.
  • Bang YJ; BK21 FOUR Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do, Republic of Korea.
  • Yoo S; Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do, Republic of Korea.
  • Park SI; SML Biopharm, Gwangmyeong, Gyeonggi-do, Republic of Korea.
  • Park HJ; Center for Brain Technology, Brain Science Institute, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.
  • Kwak HW; SML Biopharm, Gwangmyeong, Gyeonggi-do, Republic of Korea.
  • Bae SH; Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do, Republic of Korea.
  • Park HJ; BK21 FOUR Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do, Republic of Korea.
  • Kim JY; SML Biopharm, Gwangmyeong, Gyeonggi-do, Republic of Korea.
  • Youn SB; Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do, Republic of Korea.
  • Roh G; BK21 FOUR Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do, Republic of Korea.
  • Lee S; SML Biopharm, Gwangmyeong, Gyeonggi-do, Republic of Korea.
  • Kwon SP; Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do, Republic of Korea.
  • Bang EK; SML Biopharm, Gwangmyeong, Gyeonggi-do, Republic of Korea.
  • Keum G; Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do, Republic of Korea.
  • Nam JH; BK21 FOUR Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do, Republic of Korea.
  • Hong SH; Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do, Republic of Korea.
J Infect Dis ; 2023 Sep 15.
Article en En | MEDLINE | ID: mdl-37711050
Developing new adjuvants that can effectively induce both humoral and cellular immune responses while broadening the immune response is of great value. In this study, we aimed to develop GM-CSF- or IL-18-expressing single-stranded RNA (ssRNA) adjuvants based on the encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) and tested their efficacy in combination with ovalbumin (OVA) or inactivated influenza vaccines. Notably, cytokine-expressing RNA adjuvants increased the expression of antigen-presenting cell activation markers. Specifically, GM-CSF-expressing RNA adjuvants increased CD4+T cell responses, while IL-18-expressing RNA adjuvants increased CD8+T cell responses in mice when combined with OVA. In addition, cytokine-expressing RNA adjuvants increased the frequency of polyclonal T cells in combination with the influenza vaccine and reduced the clinical illness scores and weight loss of mice after viral challenge. Collectively, our results suggest that cytokine-expressing RNA adjuvants can be applied to protein-based or inactivated vaccines to increase their efficacy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Infect Dis Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Infect Dis Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos