Your browser doesn't support javascript.
loading
Construction and characterization of microsatellite markers for the Schistosoma japonicum isolate from a hilly area of China based on whole genome sequencing.
Sun, Mengtao; Cheng, Yuheng; Gao, Changzhe; Peng, Hanqi; Wang, Ning; Gu, Weiling; Lu, Dabing.
Afiliación
  • Sun M; Department of Epidemiology and Statistics, School of Public Health, Soochow University, Suzhou, China.
  • Cheng Y; Department of Epidemiology and Statistics, School of Public Health, Soochow University, Suzhou, China.
  • Gao C; Department of Epidemiology and Statistics, School of Public Health, Soochow University, Suzhou, China.
  • Peng H; Department of Epidemiology and Statistics, School of Public Health, Soochow University, Suzhou, China.
  • Wang N; Department of Epidemiology and Statistics, School of Public Health, Soochow University, Suzhou, China.
  • Gu W; Jiaxing Center for Disease Control and Prevention, Jiaxing, Zhejiang, China. jxgwl@126.com.
  • Lu D; Department of Epidemiology and Statistics, School of Public Health, Soochow University, Suzhou, China. ludabing@suda.edu.cn.
Parasitol Res ; 122(11): 2737-2748, 2023 Nov.
Article en En | MEDLINE | ID: mdl-37710024
Schistosoma japonicum had once caused the greatest disease burden in China and has still been transmitted in some hilly areas, for example, in Shitai of Anhui province, where rodents are projected to be the main reservoir. This may lead to a critical need of molecular tools with high efficiency in monitoring the dynamic of the rodent-associated S. japonicum, as an appropriate amount of schistosome input can re-establish its life cycle in a place with snails and then result in the re-emergence of schistosomiasis. Therefore, the goal of this study was to develop high polymorphic microsatellites from the whole genome of rodent-associated S. japonicum strain to monitor its transmission dynamic. We sampled the hilly schistosome isolate from Shitai of Anhui in China and sequenced the parasite with the next-generation sequencing technology. The whole genome was assembled with four different approaches. We then developed 71 microsatellite markers at a genome-wide scale throughout two best assembled genomes. Based on their chromosome mapping and the expected length of targeted sequences, we selected 24 markers for the development of multiplex reactions. Two multiplexes composed of 10 loci were finally developed, and their potential was revealed by their successful application on and capturing the genetic diversity of three schistosome populations. The selected 10 markers, each with clear chromosome location and characteristics, will be greatly useful in tracing the dispersal pathways or/and dynamics of the rodent-associated S. japonicum or others in the hilly area of China or elsewhere.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Schistosoma japonicum / Esquistosomiasis Japónica Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Parasitol Res Asunto de la revista: PARASITOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Schistosoma japonicum / Esquistosomiasis Japónica Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Parasitol Res Asunto de la revista: PARASITOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania