Your browser doesn't support javascript.
loading
The human fungal pathogen Aspergillus fumigatus can produce the highest known number of meiotic crossovers.
Auxier, Ben; Debets, Alfons J M; Stanford, Felicia Adelina; Rhodes, Johanna; Becker, Frank M; Reyes Marquez, Francisca; Nijland, Reindert; Dyer, Paul S; Fisher, Matthew C; van den Heuvel, Joost; Snelders, Eveline.
Afiliación
  • Auxier B; Laboratory of Genetics, Wageningen University; Wageningen, the Netherlands.
  • Debets AJM; Laboratory of Genetics, Wageningen University; Wageningen, the Netherlands.
  • Stanford FA; School of Life Sciences, University of Nottingham, Nottingham, United Kingdom.
  • Rhodes J; MRC Centre for Global Infectious Disease Analysis, Imperial College London, London, United Kingdom.
  • Becker FM; Laboratory of Genetics, Wageningen University; Wageningen, the Netherlands.
  • Reyes Marquez F; Laboratory of Genetics, Wageningen University; Wageningen, the Netherlands.
  • Nijland R; Marine Animal Ecology, Wageningen University, Wageningen, the Netherlands.
  • Dyer PS; School of Life Sciences, University of Nottingham, Nottingham, United Kingdom.
  • Fisher MC; MRC Centre for Global Infectious Disease Analysis, Imperial College London, London, United Kingdom.
  • van den Heuvel J; Laboratory of Genetics, Wageningen University; Wageningen, the Netherlands.
  • Snelders E; Laboratory of Genetics, Wageningen University; Wageningen, the Netherlands.
PLoS Biol ; 21(9): e3002278, 2023 09.
Article en En | MEDLINE | ID: mdl-37708139
Sexual reproduction involving meiosis is essential in most eukaryotes. This produces offspring with novel genotypes, both by segregation of parental chromosomes as well as crossovers between homologous chromosomes. A sexual cycle for the opportunistic human pathogenic fungus Aspergillus fumigatus is known, but the genetic consequences of meiosis have remained unknown. Among other Aspergilli, it is known that A. flavus has a moderately high recombination rate with an average of 4.2 crossovers per chromosome pair, whereas A. nidulans has in contrast a higher rate with 9.3 crossovers per chromosome pair. Here, we show in a cross between A. fumigatus strains that they produce an average of 29.9 crossovers per chromosome pair and large variation in total map length across additional strain crosses. This rate of crossovers per chromosome is more than twice that seen for any known organism, which we discuss in relation to other genetic model systems. We validate this high rate of crossovers through mapping of resistance to the laboratory antifungal acriflavine by using standing variation in an undescribed ABC efflux transporter. We then demonstrate that this rate of crossovers is sufficient to produce one of the common multidrug resistant haplotypes found in the cyp51A gene (TR34/L98H) in crosses among parents harboring either of 2 nearby genetic variants, possibly explaining the early spread of such haplotypes. Our results suggest that genomic studies in this species should reassess common assumptions about linkage between genetic regions. The finding of an unparalleled crossover rate in A. fumigatus provides opportunities to understand why these rates are not generally higher in other eukaryotes.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aspergillus fumigatus / Transportadoras de Casetes de Unión a ATP Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: PLoS Biol Asunto de la revista: BIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aspergillus fumigatus / Transportadoras de Casetes de Unión a ATP Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: PLoS Biol Asunto de la revista: BIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos