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ß-Glucuronidase-Activated Bioluminescence Probe for In Vivo Tumor Imaging.
Yang, Liang; Sun, Xianbao; Xu, Lingling; Cheng, Xiaotong; Liu, Xiaoyang; Deng, Yu; Hu, Xinyi; Liang, Gaolin.
Afiliación
  • Yang L; State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
  • Sun X; State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
  • Xu L; State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
  • Cheng X; State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
  • Liu X; State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
  • Deng Y; State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
  • Hu X; School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
  • Liang G; State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Anal Chem ; 95(38): 14165-14168, 2023 09 26.
Article en En | MEDLINE | ID: mdl-37702743
ß-Glucuronidase (GLU) is a hallmark enzyme for many malignant tumors, but bioluminescence (BL) probes that enable GLU imaging in vivo have not been reported. Herein, we rationally designed the BL probe Glc-Luc to address this issue. In vitro results demonstrated the specific responsiveness of Glc-Luc toward GLU with a calculated catalytic efficiency (kcat/Km) of 0.0109 µM-1 min-1 and a limit of detection (LOD) of 1.39 U/mL. Moreover, Glc-Luc rendered 3.1-fold and 15.9-fold higher BL intensities over the control groups in cell lysates and tumor-bearing mice, respectively. We anticipate that Glc-Luc could be further applied for the sensitive diagnosis of GLU-related diseases.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glucuronidasa / Neoplasias Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Anal Chem Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glucuronidasa / Neoplasias Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Anal Chem Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos