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Combined effect of DBM, PRP, and bone marrow fluid on bone union in a rat posterolateral fusion model.
Otagiri, Takuma; Shiga, Yasuhiro; Hozumi, Takashi; Matsuura, Yusuke; Tajiri, Ikuko; Takayama, Naoya; Alejandra, Maria; Shiko, Yuki; Orita, Sumihisa; Inage, Kazuhide; Eguchi, Yawara; Suzuki, Takane; Suzuki-Narita, Miyako; Mukai, Michiaki; Mukaihata, Tomohito; Tsuchiya, Ryuto; Tokeshi, Soichiro; Okuyama, Kohei; Arai, Takahito; Toshi, Noriyasu; Furuya, Takeo; Maki, Satoshi; Aoki, Yasuchika; Ohtori, Seiji.
Afiliación
  • Otagiri T; Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
  • Shiga Y; Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan. yshiga1111@yahoo.co.jp.
  • Hozumi T; Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
  • Matsuura Y; Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
  • Tajiri I; Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
  • Takayama N; Department of Regenerative Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan.
  • Alejandra M; Department of Regenerative Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan.
  • Shiko Y; Clinical Research Center, Chiba University Hospital, Chiba, Japan.
  • Orita S; Chiba University Center for Frontier Medical Engineering, Chiba, Japan.
  • Inage K; Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
  • Eguchi Y; Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
  • Suzuki T; Department of Bioenvironmental Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan.
  • Suzuki-Narita M; Department of Bioenvironmental Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan.
  • Mukai M; Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
  • Mukaihata T; Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
  • Tsuchiya R; Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
  • Tokeshi S; Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
  • Okuyama K; Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
  • Arai T; Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
  • Toshi N; Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
  • Furuya T; Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
  • Maki S; Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
  • Aoki Y; Department of Orthopaedic Surgery, Eastern Chiba Medical Center, Chiba, Japan.
  • Ohtori S; Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Sci Rep ; 13(1): 15041, 2023 09 12.
Article en En | MEDLINE | ID: mdl-37699916
Platelet-rich plasma (PRP) promotes bone union through osteoinduction. We investigated whether adding demineralized bone matrix (DBM), derived naturally from biomaterial and with various growth factors, for osteoconductivity and bone marrow fluid for osteogenesis results in different bone unions. Eight-week-old male Sprague-Dawley rats were divided into four groups of five based on transplantation material: sham control (C group); DBM alone (D group); DBM + PRP (DP group); and DBM + PRP + bone marrow fluid (DPB group). After posterolateral fusion at L3-5, postoperative weekly CT imaging determined average number of bone union in facet joints (4 joints × 5 animals = 20 joints) and bone formation. Pathological evaluation and bone strength were assessed using 3-point bending two weeks postoperatively. Facet joint bone union at four weeks postoperatively was 4/20 (20%, DP group) and 8/20 (40%, DPB group) joints. Six weeks postoperatively, it was 7/20 (35%, D group), 12/20 (60%, DP group), and 16/20 (80%, DPB group). Eight weeks postoperatively, it was 13/20 (65%, D group), 17/20 (85%, DP group), and 20/20 (100%, DPB group), suggesting that DPB > DP > D > C. Bone formation and bone strength showed a similar DPB > DP > D > C group trend. Adding PRP and bone marrow fluid to DBM promotes bone union and strength.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Líquidos Corporales / Plasma Rico en Plaquetas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Sci Rep Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Líquidos Corporales / Plasma Rico en Plaquetas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Sci Rep Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido