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Nanopore Discrimination of Nucleotide Sugars.
Wang, Kefan; Yang, Xian; Zhang, Shanyu; Zhang, Panke; Huang, Shuo.
Afiliación
  • Wang K; State Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, 210023 Nanjing, China.
  • Yang X; Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, 210023 Nanjing, China.
  • Zhang S; State Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, 210023 Nanjing, China.
  • Zhang P; Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, 210023 Nanjing, China.
  • Huang S; State Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, 210023 Nanjing, China.
Nano Lett ; 23(18): 8620-8627, 2023 09 27.
Article en En | MEDLINE | ID: mdl-37690030
Nucleotide sugars, the glycosyl donors in the biosynthesis of carbohydrates, are critical ingredients in the growth and development of all living organisms. A variety of nucleotide sugars simultaneously exist in biological samples. They, however, have only minor structural differences, which make them extremely difficult to discriminate. In this work, a phenylboronic acid (PBA)-modified Mycobacterium smegmatis porin A (MspA) hetero-octamer was applied to sense nucleotide sugars. Five representative nucleotide sugars, including guanosine diphosphate mannose (GDP-Man), adenosine diphosphate glucose (ADP-Glc), uridine diphosphate N-acetylglucosamine (UDP-GlcNAc), uridine diphosphate glucose (UDP-Glc), and uridine diphosphate glucoronic acid (UDP-GlcA), were successfully distinguished. A custom machine learning algorithm was also employed to automatically identify events, reporting a general accuracy of 99.4%. This sensing strategy provides a rapid, direct, and accurate method for identifying different nucleotide sugars. However, single-molecule identification of nucleotide sugars has never been previously reported, to the best of our knowledge.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Azúcares de Uridina Difosfato / Nanoporos Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Nano Lett Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Azúcares de Uridina Difosfato / Nanoporos Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Nano Lett Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos