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Primary Exposure to SARS-CoV-2 via Infection or Vaccination Determines Mucosal Antibody-Dependent ACE2 Binding Inhibition.
Fröberg, Janeri; Koomen, Vera J C H; van der Gaast-de Jongh, Christa E; Philipsen, Ria; GeurtsvanKessel, Corine H; de Vries, Rory D; Baas, Marije C; van der Molen, Renate G; de Jonge, Marien I; Hilbrands, Luuk B; Huynen, Martijn A; Diavatopoulos, Dimitri A.
Afiliación
  • Fröberg J; Department of Laboratory Medicine, Laboratory of Medical Immunology, Radboud University Medical Center, Nijmegen.
  • Koomen VJCH; Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Nijmegen.
  • van der Gaast-de Jongh CE; Department of Laboratory Medicine, Laboratory of Medical Immunology, Radboud University Medical Center, Nijmegen.
  • Philipsen R; Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Nijmegen.
  • GeurtsvanKessel CH; Department of Nephrology, Radboud University Medical Center, Nijmegen.
  • de Vries RD; Department of Laboratory Medicine, Laboratory of Medical Immunology, Radboud University Medical Center, Nijmegen.
  • Baas MC; Radboud Technology Center Clinical Studies, Radboud University Medical Center, Nijmegen.
  • van der Molen RG; Department of Viroscience, Erasmus Medical Center, Rotterdam.
  • de Jonge MI; Department of Viroscience, Erasmus Medical Center, Rotterdam.
  • Hilbrands LB; Department of Nephrology, Radboud University Medical Center, Nijmegen.
  • Huynen MA; Department of Laboratory Medicine, Laboratory of Medical Immunology, Radboud University Medical Center, Nijmegen.
  • Diavatopoulos DA; Department of Laboratory Medicine, Laboratory of Medical Immunology, Radboud University Medical Center, Nijmegen.
J Infect Dis ; 229(1): 137-146, 2024 Jan 12.
Article en En | MEDLINE | ID: mdl-37675756
BACKGROUND: Mucosal antibodies play a critical role in preventing SARS-CoV-2 infections or reinfections by blocking the interaction of the receptor-binding domain (RBD) with the angiotensin-converting enzyme 2 (ACE2) receptor on the cell surface. In this study, we investigated the difference between the mucosal antibody response after primary infection and vaccination. METHODS: We assessed longitudinal changes in the quantity and capacity of nasal antibodies to neutralize the interaction of RBD with the ACE2 receptor using the spike protein and RBD from ancestral SARS-CoV-2 (Wuhan-Hu-1), as well as the RBD from the Delta and Omicron variants. RESULTS: Significantly higher mucosal IgA concentrations were detected postinfection vs postvaccination, while vaccination induced higher IgG concentrations. However, ACE2-inhibiting activity did not differ between the cohorts. Regarding whether IgA or IgG drove ACE2 inhibition, infection-induced binding inhibition was driven by both isotypes, while postvaccination binding inhibition was mainly driven by IgG. CONCLUSIONS: Our study provides new insights into the relationship between antibody isotypes and neutralization by using a sensitive and high-throughput ACE2 binding inhibition assay. Key differences are highlighted between vaccination and infection at the mucosal level, showing that despite differences in the response quantity, postinfection and postvaccination ACE2 binding inhibition capacity did not differ.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: SARS-CoV-2 / COVID-19 Límite: Humans Idioma: En Revista: J Infect Dis Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: SARS-CoV-2 / COVID-19 Límite: Humans Idioma: En Revista: J Infect Dis Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos