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Lignin conversion to ß-ketoadipic acid by Pseudomonas putida via metabolic engineering and bioprocess development.
Werner, Allison Z; Cordell, William T; Lahive, Ciaran W; Klein, Bruno C; Singer, Christine A; Tan, Eric C D; Ingraham, Morgan A; Ramirez, Kelsey J; Kim, Dong Hyun; Pedersen, Jacob Nedergaard; Johnson, Christopher W; Pfleger, Brian F; Beckham, Gregg T; Salvachúa, Davinia.
Afiliación
  • Werner AZ; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA.
  • Cordell WT; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA.
  • Lahive CW; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, USA.
  • Klein BC; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA.
  • Singer CA; Catalytic Carbon Transformation and Scale-Up Center, National Renewable Energy Laboratory, Golden, CO, USA.
  • Tan ECD; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA.
  • Ingraham MA; Catalytic Carbon Transformation and Scale-Up Center, National Renewable Energy Laboratory, Golden, CO, USA.
  • Ramirez KJ; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA.
  • Kim DH; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA.
  • Pedersen JN; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA.
  • Johnson CW; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA.
  • Pfleger BF; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA.
  • Beckham GT; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, USA.
  • Salvachúa D; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA.
Sci Adv ; 9(36): eadj0053, 2023 09 08.
Article en En | MEDLINE | ID: mdl-37672573
Bioconversion of a heterogeneous mixture of lignin-related aromatic compounds (LRCs) to a single product via microbial biocatalysts is a promising approach to valorize lignin. Here, Pseudomonas putida KT2440 was engineered to convert mixed p-coumaroyl- and coniferyl-type LRCs to ß-ketoadipic acid, a precursor for performance-advantaged polymers. Expression of enzymes mediating aromatic O-demethylation, hydroxylation, and ring-opening steps was tuned, and a global regulator was deleted. ß-ketoadipate titers of 44.5 and 25 grams per liter and productivities of 1.15 and 0.66 grams per liter per hour were achieved from model LRCs and corn stover-derived LRCs, respectively, the latter representing an overall yield of 0.10 grams per gram corn stover-derived lignin. Technoeconomic analysis of the bioprocess and downstream processing predicted a ß-ketoadipate minimum selling price of $2.01 per kilogram, which is cost competitive with fossil carbon-derived adipic acid ($1.10 to 1.80 per kilogram). Overall, this work achieved bioproduction metrics with economic relevance for conversion of lignin-derived streams into a performance-advantaged bioproduct.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pseudomonas putida / Ingeniería Metabólica Tipo de estudio: Prognostic_studies Idioma: En Revista: Sci Adv Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pseudomonas putida / Ingeniería Metabólica Tipo de estudio: Prognostic_studies Idioma: En Revista: Sci Adv Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos