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Mechanism of TLR4-Mediated Anti-Inflammatory Response Induced by Exopolysaccharide from the Probiotic Bacillus subtilis.
Zamora-Pineda, Jesus; Kalinina, Olga; Sperling, Anne I; Knight, Katherine L.
Afiliación
  • Zamora-Pineda J; Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL.
  • Kalinina O; Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL.
  • Sperling AI; Pulmonary and Critical Care Division, Department of Medicine, University of Virginia, Charlottesville, VA.
  • Knight KL; Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL.
J Immunol ; 211(8): 1232-1239, 2023 10 15.
Article en En | MEDLINE | ID: mdl-37672039
Intestinal inflammatory diseases affect millions of people worldwide, and one class of drugs showing promise toward treatment of several inflammatory diseases is probiotics. Numerous studies have been performed using probiotics to prevent and treat intestinal inflammatory diseases. Most of these studies used intact bacteria, and neither the active molecule nor the molecular mechanisms by which they affect immune responses are known. We have shown that the probiotic Bacillus subtilis is anti-inflammatory and can protect mice from acute colitis induced by the enteric pathogen Citrobacter rodentium. We identified and purified the active molecule, exopolysaccharide (EPS), and showed that it protects mice from C. rodentium-induced colitis by inducing anti-inflammatory M2 macrophages or inhibitory dendritic cells (DCs), both of which inhibit excessive T cell responses. We showed previously that EPS affects macrophages and DCs in a TLR4-dependent manner, and in the current study we asked how EPS induces these anti-inflammatory cells and how they function to inhibit T cells. By investigating the signaling downstream of TLR4 that leads to acquisition of inhibitory properties of macrophages and DCs, we found that EPS induces expression of the inhibitory molecule IDO in bone marrow-derived DCs, and that inhibition of T cell proliferation by IDO-expressing bone marrow-derived DCs utilizes the kynurenine/aryl hydrocarbon receptor circuit. Furthermore, unlike LPS, EPS does not induce inflammatory cytokines upon injection in vivo, directly demonstrating different outcomes induced by two different TLR4 agonists.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colitis / Probióticos Límite: Animals / Humans Idioma: En Revista: J Immunol Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colitis / Probióticos Límite: Animals / Humans Idioma: En Revista: J Immunol Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos