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Vitrification induces a focused spindle pole in mouse MI oocytes.
Guo, Yaoyao; Sun, Haowei; Chen, Hui; Yang, Gongshe; Wang, Jialun; Qi, Zhengjun; Pang, Weijun; Chu, Guiyan; Gao, Lei.
Afiliación
  • Guo Y; Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: GYY19981111@163.com.
  • Sun H; Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: shwwj325@163.com.
  • Chen H; Animal Husbandry Industry Test and Demonstration Center of Shaanxi Province, Jingyang, 713708, Shaanxi, China. Electronic address: chenhui089@163.com.
  • Yang G; Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: gsyang999@hotmail.com.
  • Wang J; Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: 1067875173@qq.com.
  • Qi Z; Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: qizj2001@163.com.
  • Pang W; Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: pwj1226@nwafu.edu.cn.
  • Chu G; Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: guiyanchu@nwafu.edu.cn.
  • Gao L; Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: gaolei5414@nwafu.edu.cn.
Theriogenology ; 211: 232-240, 2023 Nov.
Article en En | MEDLINE | ID: mdl-37660475
Immature oocyte (germinal vesicle stage, GV) vitrification can avoid a cycle of ovarian stimulation, which is friendly to patients with hormone-sensitive tumors. However, the in vitro maturation of vitrification-thawed GV oocyte usually results in aneuploidy, and the underlying mechanism remains unclear. Stable spindle poles are important for accurate chromosome segregation. Acentriolar microtubule-organizing centers (aMTOCs) undergo fragmentation and reaggregation to form spindle poles. Microtubule nucleation is facilitated via the perichromosome Ran after GVBD, which plays an important role in aMTOCs fragmentation. This study showed that vitrification may reduce microtubule density by decreasing perichromosomal Ran levels, which reduced the localization of pKIF11, thereby decreased the fragmentation of aMTOCs and formed a more focused spindle pole, ultimately resulted in aneuploidy. This study revealed the mechanism of abnormal spindle pole formation in vitrified oocytes and offered a theoretical support to further improve the quality of vitrified oocytes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vitrificación Límite: Animals Idioma: En Revista: Theriogenology Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vitrificación Límite: Animals Idioma: En Revista: Theriogenology Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos