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Development of Genetically Engineered Feeder Cells for Natural Killer Cell Expansion.
Lee, DA Yeon; Kim, Yeongrin; Park, Jin Song; Choi, Ji U; Jeong, Hye Gwang; Park, Chi Hoon.
Afiliación
  • Lee DY; Bio & Drug Discovery Division, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea.
  • Kim Y; College of Pharmacy, Chungnam National University, Daejeon, Republic of Korea.
  • Park JS; Bio & Drug Discovery Division, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea.
  • Choi JU; Medicinal Chemistry and Pharmacology, Korea University of Science and Technology, Daejeon, Republic of Korea.
  • Jeong HG; Bio & Drug Discovery Division, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea.
  • Park CH; Bio & Drug Discovery Division, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea.
Anticancer Res ; 43(9): 3897-3904, 2023 Sep.
Article en En | MEDLINE | ID: mdl-37648291
BACKGROUND/AIM: To obtain sufficient numbers of high-quality natural killer (NK) cells, we developed feeder cells using synthetic biology techniques. MATERIALS AND METHODS: K562 cells were engineered to express membrane bound interleukin-2 (mbIL2) or interleukin-13 (mbIL13). RESULTS: The incubation of human primary NK cells isolated from peripheral blood mononuclear cells (PBMCs) with these feeder cells significantly increased the number of activated NK cells compared to K562 parental cells. Fluorescence-activated cell sorting (FACS) analysis demonstrated that NKG2D activating receptors were abundant on the surface of NK cells expanded by K562-mbIL2 or mbIL13 cells. NK cells expanded on K562-mbIL2 or mbIL13 lysed cancer cells more effectively than those cultured with normal K562 cells. Using NK cells incubated with our feeder cells, we developed anti-CD19 chimeric antigen receptor (CAR)-NK cells. They showed robust cytotoxic effect against CD19 positive cancer cell line. CONCLUSION: Our newly developed feeder cells could provide useful tools for NK cell therapy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucocitos Mononucleares / Células Asesinas Naturales Límite: Humans Idioma: En Revista: Anticancer Res Año: 2023 Tipo del documento: Article Pais de publicación: Grecia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucocitos Mononucleares / Células Asesinas Naturales Límite: Humans Idioma: En Revista: Anticancer Res Año: 2023 Tipo del documento: Article Pais de publicación: Grecia