Your browser doesn't support javascript.
loading
Hsa_circ_0015382 is involved in the pathogenesis of preeclampsia by mediating THBS2 expression.
Wang, Yang; Liu, Lingfang; Wang, Jiayao; Gao, Yan.
Afiliación
  • Wang Y; Department of Obstetrics, Sichuan Provincial Maternity and Child Health Care Hospital, The Affiliated Women's and Children's Hospital of Chengdu Medical College, Chengdu, Sichuan Province, China.
  • Liu L; Department of Obstetrics, Sichuan Provincial Maternity and Child Health Care Hospital, The Affiliated Women's and Children's Hospital of Chengdu Medical College, Chengdu, Sichuan Province, China.
  • Wang J; School of Clinical Medicine, Chengdu Medical College, Chengdu, Sichuan Province, China.
  • Gao Y; Department of Obstetrics, Sichuan Provincial Maternity and Child Health Care Hospital, The Affiliated Women's and Children's Hospital of Chengdu Medical College, Chengdu, Sichuan Province, China.
Am J Reprod Immunol ; 90(3): e13760, 2023 09.
Article en En | MEDLINE | ID: mdl-37641374
BACKGROUND: Preeclampsia (PE) is a hypertensive disorder of pregnancy that causes significant maternal and perinatal morbidity and mortality. Circular RNA (circRNA) hsa_circ_0015382 is associated with the pathogenesis of PE, but its underlying regulatory mechanism remains to be explored. METHODS: Relative RNA levels of hsa_circ_0015382, microRNA-616-3p and thrombospondin-2 (THBS2) were detected by quantitative reverse transcription-polymerase chain reaction. In vitro regulatory effects of hsa_circ_0015382 on the proliferation, migration, invasion and angiogenesis of trophoblasts were evaluated by CCK-8, flow cytometry for cell cycle, EdU, transwell, wound healing and HUVEC tube formation assays, respectively. Targeting interaction was verified by dual-luciferase reporter and RNA immunoprecipitation assays. RESULTS: Hsa_circ_0015382 was highly expressed in placental tissues from PE patients. Upregulation of hsa_circ_0015382 repressed trophoblast proliferation, migration, invasion and lowered trophoblast-induced HUVEC tube formation. Hsa_circ_0015382 was validated as a miR-616-3p sponge and miR-616-3p targeted THBS2. Hsa_circ_0015382 could mediate trophoblast proliferation, migration, invasion and regulate trophoblast-induced HUVEC tube formation by sponging miR-616-3p and regulating THBS2 expression. CONCLUSION: Hsa_circ_0015382 is associated with the pathogenesis of PPE by regulating the miR-616-3p/THBS2 axis. HIGHLIGHTS: Hsa_circ_0015382 is overexpressed in preeclampsia patients. Hsa_circ_0015382 inhibits trophoblast proliferation, migration, invasion and decreases trophoblast-induced HUVEC tube formation. Hsa_circ_0015382 interacts with miR-616-3p to regulate THBS2 expression.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preeclampsia / MicroARNs / Hipertensión Tipo de estudio: Etiology_studies Límite: Female / Humans / Pregnancy Idioma: En Revista: Am J Reprod Immunol Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Dinamarca
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preeclampsia / MicroARNs / Hipertensión Tipo de estudio: Etiology_studies Límite: Female / Humans / Pregnancy Idioma: En Revista: Am J Reprod Immunol Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Dinamarca