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Using Gamma Irradiation to Predict Sperm Competition Mechanism in Bagrada hilaris (Burmeister) (Hemiptera: Pentatomidae): Insights for a Future Management Strategy.
Mainardi, Chiara Elvira; Peccerillo, Chiara; Paolini, Alessandra; Cemmi, Alessia; Sforza, René F H; Musmeci, Sergio; Porretta, Daniele; Cristofaro, Massimo.
Afiliación
  • Mainardi CE; Biotechnology and Biological Control Agency (BBCA) Onlus, Via Angelo Signorelli 105, 00123 Rome, Italy.
  • Peccerillo C; Department of Environmental Biology, University of Rome "La Sapienza", 00185 Rome, Italy.
  • Paolini A; Center of Agriculture, Food and Environment (C3A), University of Trento, 38010 San Michele all'Adige, Italy.
  • Cemmi A; Biotechnology and Biological Control Agency (BBCA) Onlus, Via Angelo Signorelli 105, 00123 Rome, Italy.
  • Sforza RFH; FSN-FISS-SNI Laboratory, Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), Via Anguillarese 301, 00123 Rome, Italy.
  • Musmeci S; European Biological Control Laboratory, (USDA-ARS-EBCL), United States Department of Agriculture, 810 Avenue du Campus Agropolis, 34980 Montferrier-sur-Lez, France.
  • Porretta D; SSPT-BIOAG-SOQUAS Laboratory, Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), Via Anguillarese 301, 00123 Rome, Italy.
  • Cristofaro M; Department of Environmental Biology, University of Rome "La Sapienza", 00185 Rome, Italy.
Insects ; 14(8)2023 Aug 01.
Article en En | MEDLINE | ID: mdl-37623391
The stink bug, Bagrada hilaris, is a pest of mainly Brassicaceae crops. It is native to Africa and Asia and was recently reported as invasive in the southwestern part of the USA and in South America. There are no mitigation programs in place that do not involve pesticides. Therefore, much attention has recently been paid to the study of this species in order to identify sustainable and effective control strategies, such as the Sterile Insect Technique (SIT). In order to evaluate the suitability of the SIT on this pest, the mechanism of post-copulatory sperm competition was investigated. This is a polyandrous species, and it is thus important to understand whether irradiated males are able to compete with wild, e.g., non-irradiated, males for sperm competition after matings. Sperm competition was studied by sequentially mating a healthy virgin female first with a non-irradiated male, and then with a γ-irradiated (Co-60) one, and again in the opposite order. Males were irradiated at three different doses: 60, 80, and 100 Gy. The fecundity and fertility of the females, in the two orders of mating, were scored in order to perform an initial assessment of the success of sperm competition with a P2 index. Sperm from the non-irradiated male were utilized at the lowest irradiation doses (60 and 80 Gy), whereas the irradiated sperm were preferentially utilized at the highest dose (100 Gy). Bagrada hilaris exhibited high variability in P2 indexes, indicating a sperm-mixing mechanism.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Insects Año: 2023 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Insects Año: 2023 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza