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The minus-end depolymerase KIF2A drives flux-like treadmilling of γTuRC-uncapped microtubules.
Henkin, Gil; Brito, Cláudia; Thomas, Claire; Surrey, Thomas.
Afiliación
  • Henkin G; Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST) , Barcelona, Spain.
  • Brito C; Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST) , Barcelona, Spain.
  • Thomas C; The Francis Crick Institute , London, UK.
  • Surrey T; Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST) , Barcelona, Spain.
J Cell Biol ; 222(10)2023 10 02.
Article en En | MEDLINE | ID: mdl-37615667
During mitosis, microtubules in the spindle turn over continuously. At spindle poles, where microtubule minus ends are concentrated, microtubule nucleation and depolymerization, the latter required for poleward microtubule flux, happen side by side. How these seemingly antagonistic processes of nucleation and depolymerization are coordinated is not understood. Here, we reconstitute this coordination in vitro combining different pole-localized activities. We find that the spindle pole-localized kinesin-13 KIF2A is a microtubule minus-end depolymerase, in contrast to its paralog MCAK. Due to its asymmetric activity, KIF2A still allows microtubule nucleation from the γ-tubulin ring complex (γTuRC), which serves as a protective cap shielding the minus end against KIF2A binding. Efficient γTuRC uncapping requires the combined action of KIF2A and a microtubule severing enzyme, leading to treadmilling of the uncapped microtubule driven by KIF2A. Together, these results provide insight into the molecular mechanisms by which a minimal protein module coordinates microtubule nucleation and depolymerization at spindle poles consistent with their role in poleward microtubule flux.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cinesinas / Centro Organizador de los Microtúbulos Límite: Humans Idioma: En Revista: J Cell Biol Año: 2023 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cinesinas / Centro Organizador de los Microtúbulos Límite: Humans Idioma: En Revista: J Cell Biol Año: 2023 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos