Your browser doesn't support javascript.
loading
Identification of an Acinetobacter pittii acyltransferase involved in transformation of deoxynivalenol to 3-acetyl-deoxynivalenol by transcriptomic analysis.
Liu, Yuxuan; Xu, Laipeng; Shi, Ziyao; Wang, Ruolin; Liu, Yang; Gong, Yujie; Tian, Yadong; Kang, Xiangtao; Sun, Xiangli; Wang, Yanbin.
Afiliación
  • Liu Y; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, People's Republic of China.
  • Xu L; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, People's Republic of China.
  • Shi Z; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, People's Republic of China.
  • Wang R; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, People's Republic of China.
  • Liu Y; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, People's Republic of China.
  • Gong Y; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450000, People's Republic of China.
  • Tian Y; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450000, People's Republic of China.
  • Kang X; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450000, People's Republic of China.
  • Sun X; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450000, People's Republic of China. Electronic address: xlsun1996@henau.edu.cn.
  • Wang Y; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, People's Republic of China. Electronic address: ybwang2008@henau.edu.cn.
Ecotoxicol Environ Saf ; 263: 115395, 2023 Sep 15.
Article en En | MEDLINE | ID: mdl-37611475
Deoxynivalenol (DON), a mycotoxin primarily produced by Fusarium graminearum (F. graminearum), is widely present in food and feed, posing great hazards to human and livestock health. In this study, a strain of Acinetobacter pittii (A. pittii) S12 capable of degrading DON was isolated from soil samples and identified through morphological characterization, biochemistry analysis, and 16 S rRNA gene sequencing. The results of HPLC-MS indicated that the degradation products underwent a conversion from [M-H]- to [M+CH3CO], with concomitant transformation of the hydroxyl group into an acetyl moiety. Based on transcriptome sequencing analysis, the acyltransferase encoded by DLK06_RS13370 was predicted to be the pivotal gene responsible for DON biotransformation. The result of molecular docking analysis suggest a high affinity between the enzyme and DON. The recombinant protein encoded by DLK06_RS13370 was expressed in Escherichia coli (E. coli) and demonstrated the capacity to catalyze the conversion of DON into 3-Acetyl-deoxynivalenol (3-ADON), as confirmed by HPLC analysis. In conclusion, our findings confirm that the acyltransferase encoded by DLK06-RS13370 is responsible for the acetylation of DON. This sheds light on the co-occurrence of DON and its acetyl-derivatives in wheat-based products. DATA AVAILABILITY: Not applicable.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aciltransferasas / Escherichia coli Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: Ecotoxicol Environ Saf Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aciltransferasas / Escherichia coli Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: Ecotoxicol Environ Saf Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos