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Cryo-electron microscopy structure and translocation mechanism of the crenarchaeal ribosome.
Wang, Ying-Hui; Dai, Hong; Zhang, Ling; Wu, Yun; Wang, Jingfen; Wang, Chen; Xu, Cai-Huang; Hou, Hai; Yang, Bing; Zhu, Yongqun; Zhang, Xing; Zhou, Jie.
Afiliación
  • Wang YH; Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang 310058, China.
  • Dai H; Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang 310058, China.
  • Zhang L; Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang 310058, China.
  • Wu Y; Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang 310058, China.
  • Wang J; Center for Cryo-Electron Microscopy, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.
  • Wang C; Department of Pathology of Sir Run Run Shaw Hospital, and Department of Biophysics, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.
  • Xu CH; Center for Cryo-Electron Microscopy, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.
  • Hou H; Department of Pathology of Sir Run Run Shaw Hospital, and Department of Biophysics, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.
  • Yang B; Center for Cryo-Electron Microscopy, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.
  • Zhu Y; Department of Pathology of Sir Run Run Shaw Hospital, and Department of Biophysics, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.
  • Zhang X; Institute of Medical Research, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China.
  • Zhou J; Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang 310058, China.
Nucleic Acids Res ; 51(17): 8909-8924, 2023 09 22.
Article en En | MEDLINE | ID: mdl-37604686
Archaeal ribosomes display variations in their ribosomal proteins and ribosomal RNA (rRNA) expansion segments (ESs). Protein translation in archaea combines features in both bacterial and eukaryotic translation. In this study, we present 10 cryo-electron microscopy structures of the archaeal ribosome from crenarchaeota Sulfolobus acidocaldarius (Sac). The 50S and 30S subunit structures present 3 novel ribosomal proteins and 12 rRNA ESs. The 70S Sac ribosome structures were captured in seven distinct functional states, including pre-, intermediate- and post-translocation states. Specifically, we identified two novel translocation intermediates, in which the 30S subunit head domain tilts outward to release the translocated P-site transfer RNA. The structures of archaeal ribosomes provide insights into the archaeal translation and ribosome translocation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ribosomas / Sulfolobus acidocaldarius Idioma: En Revista: Nucleic Acids Res Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ribosomas / Sulfolobus acidocaldarius Idioma: En Revista: Nucleic Acids Res Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido