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Comprehensive Molecular Characterization of Polymorphous Adenocarcinoma, Cribriform Subtype: Identifying Novel Fusions and Fusion Partners.
Hahn, Elan; Xu, Bin; Katabi, Nora; Dogan, Snjezana; Smith, Stephen M; Perez-Ordonez, Bayardo; Patel, Paras B; MacMillan, Christina; Lubin, Daniel J; Gagan, Jeffrey; Weinreb, Ilan; Bishop, Justin A.
Afiliación
  • Hahn E; Department of Pathology and Laboratory Medicine, Sinai Health System, Toronto, Ontario, Canada; Department of Laboratory Medicine and Pathobiology, University of Toronto, Ontario, Canada. Electronic address: elan.hahn@sinaihealth.ca.
  • Xu B; Department of Pathology and Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Katabi N; Department of Pathology and Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Dogan S; Department of Pathology and Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Smith SM; Department of Laboratory Medicine and Pathobiology, University of Toronto, Ontario, Canada; Laboratory Medicine Program, University Health Network, Toronto General Hospital, Toronto, Ontario, Canada.
  • Perez-Ordonez B; Department of Laboratory Medicine and Pathobiology, University of Toronto, Ontario, Canada; Laboratory Medicine Program, University Health Network, Toronto General Hospital, Toronto, Ontario, Canada.
  • Patel PB; ProPath, Dallas, Texas.
  • MacMillan C; Department of Pathology and Laboratory Medicine, Sinai Health System, Toronto, Ontario, Canada; Department of Laboratory Medicine and Pathobiology, University of Toronto, Ontario, Canada.
  • Lubin DJ; Department of Pathology and Laboratory Medicine, Emory University Hospital, Atlanta, Georgia.
  • Gagan J; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas.
  • Weinreb I; Department of Laboratory Medicine and Pathobiology, University of Toronto, Ontario, Canada; Laboratory Medicine Program, University Health Network, Toronto General Hospital, Toronto, Ontario, Canada.
  • Bishop JA; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas.
Mod Pathol ; 36(11): 100305, 2023 11.
Article en En | MEDLINE | ID: mdl-37595638
Polymorphous adenocarcinoma (PAC) is a common, usually low-grade salivary gland carcinoma. While conventional PACs are most associated with PRKD1 p.E710D hotspot mutations, the cribriform subtype is often associated with gene fusions in PRKD1, PRKD2, or PRKD3. These fusions have been primarily identified by fluorescence in situ hybridization (FISH) analysis, with a minority evaluated by next-generation sequencing (NGS). Many of the reported fusions were detected by break-apart FISH probes and therefore have unknown partners or were negative by FISH altogether. In this study, we aimed to further characterize the fusions associated with PAC with NGS. Fifty-four PACs (exclusively cribriform and mixed/intermediate types to enrich the study for fusion-positive cases) were identified and subjected to NGS. Fifty-one cases were successfully sequenced, 28 of which demonstrated gene fusions involving PRKD1, PRKD2, or PRKD3. There were 10 cases with the PRKD1 p.E710D mutation. We identified a diverse group of fusion partners, including 13 novel partners, 3 of which were recurrent. The most common partners for the PRKD genes were ARID1A and ARID1B. The wide variety of involved genes is unlike in other salivary gland malignancies and warrants a broader strategy of sequencing for molecular confirmation for particularly challenging cases, as our NGS study shows.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de las Glándulas Salivales / Adenocarcinoma Límite: Humans Idioma: En Revista: Mod Pathol Asunto de la revista: PATOLOGIA Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de las Glándulas Salivales / Adenocarcinoma Límite: Humans Idioma: En Revista: Mod Pathol Asunto de la revista: PATOLOGIA Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos