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Quantifying Cell Death Induced by the NLRC4 Inflammasome.
Emming, Stefan; Monteleone, Mercedes M; Kambara, Hiroto; Starchenko, Alina; Alley, Jennifer; Nolan, Michael A; Li, Wei; Kilty, Iain; Schroder, Kate.
Afiliación
  • Emming S; Institute for Molecular Bioscience, and Centre for Inflammation and Disease Research, The University of Queensland, St. Lucia, Australia.
  • Monteleone MM; Institute for Molecular Bioscience, and Centre for Inflammation and Disease Research, The University of Queensland, St. Lucia, Australia.
  • Kambara H; Quench Bio, Inc., Cambridge, MA, USA.
  • Starchenko A; Quench Bio, Inc., Cambridge, MA, USA.
  • Alley J; Quench Bio, Inc., Cambridge, MA, USA.
  • Nolan MA; Quench Bio, Inc., Cambridge, MA, USA.
  • Li W; Quench Bio, Inc., Cambridge, MA, USA.
  • Kilty I; Quench Bio, Inc., Cambridge, MA, USA.
  • Schroder K; Institute for Molecular Bioscience, and Centre for Inflammation and Disease Research, The University of Queensland, St. Lucia, Australia. k.schroder@imb.uq.edu.au.
Methods Mol Biol ; 2696: 199-210, 2023.
Article en En | MEDLINE | ID: mdl-37578724
The Nod-like Receptor (NLR) apoptosis inhibitory proteins (NAIPs) are cytosolic receptors that sense cytosolic bacterial proteins. NAIP ligation induces its association with NLRC4, leading to the assembly of the NAIP/NLRC4 inflammasome, which induces the activation of the caspase-1 protease. Caspase-1 then cleaves pro-interleukin (IL)-1ß, pro-IL-18, and gasdermin D and induces a form of pro-inflammatory cell death, pyroptosis. These processes culminate in host defense against bacterial infection. Here we describe methods for activating NAIP/NLRC4 inflammasome signalling in human and murine macrophages and quantifying inflammasome-induced cell death.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Unión al Calcio / Inflamasomas Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Unión al Calcio / Inflamasomas Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos