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Ultra-sensitive photoelectrochemical biosensor for determination of African swine fever virus based on surface plasmon resonance.
Yuan, Ruishuang; Wei, Jie; Geng, Rui; Li, Bin; Xiong, Wei; Fang, Xueen; Lü, Peng; Wang, Kun.
Afiliación
  • Yuan R; Key Laboratory of Modern Agricultural Equipment and Technology (Jiangsu University), Ministry of Education, School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, PR China.
  • Wei J; School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
  • Geng R; School of Life Sciences, Jiangsu University, Zhenjiang, 212003, PR China.
  • Li B; Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
  • Xiong W; Technical Center for Animal, Plant and Food Inspection and Quarantine of Shanghai Customs, 1208 Minsheng Road, Pudong District, Shanghai, 200135, PR China.
  • Fang X; Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, 200433, PR China.
  • Lü P; School of Life Sciences, Jiangsu University, Zhenjiang, 212003, PR China. Electronic address: penglu@ujs.edu.cn.
  • Wang K; Key Laboratory of Modern Agricultural Equipment and Technology (Jiangsu University), Ministry of Education, School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, PR China; School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China. Electronic add
Anal Chim Acta ; 1276: 341637, 2023 Oct 02.
Article en En | MEDLINE | ID: mdl-37573117
Sensitive and specific detection of African swine fever virus (ASFV) is crucial for agricultural production and economic development due to the mortality and infectivity. In this study, a bismuth induced enhanced photoelectrochemical (PEC) biosensor based on in-situ loop mediated isothermal amplification (LAMP) was constructed using deposited bismuth nanoparticles loaded bismuth oxycarbonate (Bi/(BiO)2CO3) as photoactive material, using primers designed according to LAMP as recognition elements, and using in-situ LAMP to achieve nucleic acid amplification of target genes. As the Bi induced surface plasmon resonance (SPR) effect, enhanced light captures and effective electron hole separation, it could effectively enhance the photoelectric activity, so the prepared Bi/(BiO)2CO3 nanohybrid had higher photocurrent intensity and good stability. The constructed PEC biosensor has realized the detection of ASFV in real samples with good sensitivity, specificity and repeatability. In the range from 1.0 × 10-13 to 1.0 × 10-7 g/L, the photoelectric current decreased with the increase of the concentration of ASFV, and the detection limit was 3.0 × 10-14 g/L (about 0.048 copies/µL). Combining the advantages of LAMP with the excellent performance of PEC, it provides a simple, economical and efficient method for nucleic acid diagnosis, and also provides a new idea for biosensor detection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Chim Acta Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Chim Acta Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos