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Metagenomic insights into microbial contamination in critical healthcare environments and the efficacy of a novel "HLE" disinfectant.
Abriouel, Hikmate; Manetsberger, Julia; Lavilla Lerma, Leyre; Pestaña Blanco, María Dolores; Martínez Nogueras, Rafael; Caballero Gómez, Natacha; Benomar, Nabil.
Afiliación
  • Abriouel H; Área de Microbiología, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentals, Universidad de Jaén, 23071, Jaén, Spain. Electronic address: hikmate@ujaen.es.
  • Manetsberger J; Área de Microbiología, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentals, Universidad de Jaén, 23071, Jaén, Spain.
  • Lavilla Lerma L; Área de Microbiología, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentals, Universidad de Jaén, 23071, Jaén, Spain.
  • Pestaña Blanco MD; Hospital Universitario de Jaén, Spain.
  • Martínez Nogueras R; Hospital Universitario de Jaén, Spain.
  • Caballero Gómez N; Área de Microbiología, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentals, Universidad de Jaén, 23071, Jaén, Spain.
  • Benomar N; Área de Microbiología, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentals, Universidad de Jaén, 23071, Jaén, Spain.
Infect Dis Health ; 28(4): 282-289, 2023 11.
Article en En | MEDLINE | ID: mdl-37558566
BACKGROUND: Bacterial contamination on inanimate clinical surfaces is directly linked to severe health problems, especially those caused by multidrug resistant (MDR) pathogens. Here, we evaluated the microbial burden in these environments and tested the efficacy of a novel HLE disinfectant solution. METHODS: Microbial contamination of healthcare surfaces [Intensive Care Unit (ICU), Long Period Hospitalization Room (LPHR) and Otolaryngology Consultation (OC)] and the efficacy of HLE disinfectant solution were determined analyzing the viable counts on general and selective media, and also by molecular studies focused on metagenomic and specific qPCR. RESULTS: Different contamination loads were detected with LPHR showing the highest contamination. Treatment with the HLE disinfectant solution curbed the spread of well-adapted pathogens on touched surfaces (ICU, LPHR and OC). Metagenomic analysis of microbial diversity of the Patient Table (most contaminated surface in LPHR) revealed the presence of mainly A. johnsonii and P. putida. Furthermore, functional annotation of toxin, virulence and antibiotic resistance sequences showed a high diversity of Acinetobacter spp. and Pseudomonas spp. In this context, specific qPCR analysis confirmed the efficacy of HLE disinfectant solution against the most prevalent and critical pathogens Pseudomonas sp. and Acinetobacter sp. achieving their complete eradication. CONCLUSION: Given the persistence of detrimental resistant pathogens, the application of HLE disinfection solution could be a highly beneficial and effective option -used either alone or in combination-for infection prevention and control with the aim to eliminate microbial pathogens and their genes from contaminated contact-surfaces and thus limit the spread to humans and other ecological niches.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Desinfectantes Límite: Humans Idioma: En Revista: Infect Dis Health Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Desinfectantes Límite: Humans Idioma: En Revista: Infect Dis Health Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos