Your browser doesn't support javascript.
loading
Enhanced fluorescence detection of miRNA by means of Bloch surface wave-based biochips.
Occhicone, Agostino; Michelotti, Francesco; Rosa, Paola; Chiappetta, Daniele; Pileri, Tommaso; Del Porto, Paola; Danz, Norbert; Munzert, Peter; Pignataro, Giuseppe; Sinibaldi, Alberto.
Afiliación
  • Occhicone A; SAPIENZA Università di Roma, Department of Basic and Applied Sciences for Engineering, Via A. Scarpa, 16, 00161 Roma, Italy. agostino.occhicone@uniroma1.it.
  • Michelotti F; Center for Life Nano and Neuro Science, Istituto Italiano di Tecnologia (IIT), Viale Regina Elena 291, 00161 Rome, Italy.
  • Rosa P; SAPIENZA Università di Roma, Department of Basic and Applied Sciences for Engineering, Via A. Scarpa, 16, 00161 Roma, Italy. agostino.occhicone@uniroma1.it.
  • Chiappetta D; Center for Life Nano and Neuro Science, Istituto Italiano di Tecnologia (IIT), Viale Regina Elena 291, 00161 Rome, Italy.
  • Pileri T; SAPIENZA Università di Roma, Department of Basic and Applied Sciences for Engineering, Via A. Scarpa, 16, 00161 Roma, Italy. agostino.occhicone@uniroma1.it.
  • Del Porto P; SAPIENZA Università di Roma, Department of Basic and Applied Sciences for Engineering, Via A. Scarpa, 16, 00161 Roma, Italy. agostino.occhicone@uniroma1.it.
  • Danz N; Center for Life Nano and Neuro Science, Istituto Italiano di Tecnologia (IIT), Viale Regina Elena 291, 00161 Rome, Italy.
  • Munzert P; SAPIENZA Università di Roma, Department of Basic and Applied Sciences for Engineering, Via A. Scarpa, 16, 00161 Roma, Italy. agostino.occhicone@uniroma1.it.
  • Pignataro G; Department of Biology and Biotechnology "C. Darwin", Sapienza University of Rome, 00185 Rome, Italy.
  • Sinibaldi A; Fraunhofer Institute for Applied Optics and Precision Engineering, A.-Einstein-Str. 7, 07745 Jena, Germany.
Analyst ; 148(18): 4429-4437, 2023 Sep 11.
Article en En | MEDLINE | ID: mdl-37555461
We report on the use of biochips based on one-dimensional photonic crystals sustaining Bloch surface waves to specifically detect target miRNA that is characteristic of hemorrhagic stroke (miR-16-5p) at low concentration in a buffer solution. The biochips were functionalized with streptavidin and ssDNA oligonucleotides to enable miRNA detection. To discriminate the target miRNA from a non-specific control (miR-101a-3p), we made use of an optical platform developed to work both in label-free and fluorescence detection modes. We demonstrate that the limit of detection provided when operating in the fluorescence mode allows us to specifically detect the target miRNA down to 1 ng mL-1 (140 pM), which matches the recommendations for diagnostic miRNA assays, 5 ng mL-1. The low costs open the way towards the application of these disposable optical biochips based on 1DPC sustaining Bloch surface waves as a promising tool for early disease detection in a liquid biopsy format.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs Tipo de estudio: Diagnostic_studies / Guideline Idioma: En Revista: Analyst Año: 2023 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs Tipo de estudio: Diagnostic_studies / Guideline Idioma: En Revista: Analyst Año: 2023 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido